Details and Advantages
Applications:
ICC/IF,Flow Cyt
Reactivity:
Mouse IgG3(Fcγ fragment specific)
Conjugate:
APC
Advantages:
Consistent and reproducible results;
· Extraordinarybinding,even under harsh conditions;
· High affinity and Short incubation (5-30min)
Summary
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Description:
Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(APC) is designed for detecting mouse IgG3 Fcγ fragment specifically. Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(APC) is based on recombinant single domain antibody to mouse IgG3 Fc coupled to APC. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(APC) reacts with the Fc fragment of mouse IgG3 selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Immunogen: Recombinant Fc region of mouse IgG3
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: APC(Ex: 651nm, Em: 662nm)
Specificity: Mouse IgG3(Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgG1/2a/2b, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.1mg/mL
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH.
Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(APC) is designed for detecting mouse IgG3 Fcγ fragment specifically. Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(APC) is based on recombinant single domain antibody to mouse IgG3 Fc coupled to APC. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(APC) reacts with the Fc fragment of mouse IgG3 selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Immunogen: Recombinant Fc region of mouse IgG3
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: APC(Ex: 651nm, Em: 662nm)
Specificity: Mouse IgG3(Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgG1/2a/2b, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.1mg/mL
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH.
Performance
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Flow Cyt: 1:200-1:1000
ICC/IF： 1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ICC/IF： 1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
