Anti-Mouse IgG(H+L), AlpSdAbs® VHH(APC)

Details and Advantages

                                                Applications:
                                                ICC/IF,Flow Cyt
                                            
                                                Reactivity:
                                                Mouse IgG(H+L)
                                            
                                                Conjugate:
                                                APC
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-Mouse IgG(H+L), AlpSdAbs® VHH(APC) is designed for detecting mouse IgG(H+L) specifically, and Anti-Mouse IgG(H+L), AlpSdAbs® VHH(APC) is useful for super-resolution microscopy. Anti-Mouse IgG(H+L), AlpSdAbs® VHH(APC) is based on recombinant single domain antibodies to mouse IgG(H+L) coupled to APC. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG(H+L), AlpSdAbs® VHH(APC) detects the heavy chain and light chain of mouse IgG selectively, no reactivity with rabbit, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: APC(Ex: 651nm, Em: 662nm)
Specificity: Mouse IgG(H+L)
Cross-Reactivity: No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.1mg/mL
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH.

Performance >

Flow Cyt: 1:200-1:1000
ICC/IF： 1:200-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.