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Anti-Sheep IgG(H+L), AlpSdAbs® VHH(APC)

Details and Advantages
Applications: ICC/IF,Flow Cyt
Reactivity: Sheep IgG
Conjugate: APC
Advantages:

Consistent and reproducible results;

· Extraordinarybinding,even under harsh conditions;

· High affinity and Short incubation (5-30min)

Summary >
Description:
Anti- Sheep IgG(H+L), AlpSdAbs® VHH(APC) is designed for detecting sheep IgG specifically. Anti- Sheep IgG(H+L), AlpSdAbs® VHH(APC) is based on recombinant single domain antibodies to sheep IgG coupled to APC. Based on immunoelectrophoresis and/or ELISA, Anti- Sheep IgG(H+L), AlpSdAbs® VHH(APC) reacts with the Sheep IgG selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat IgG.

Immunogen: Sheep IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: APC(Ex: 651nm, Em: 662nm)
Specificity: Sheep IgG
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance >
Flow Cyt: 1:200-1:1000
ICC/IF: 1:200-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.