Have you ever encountered these problems when performing IP/Co-IP (immunoprecipitation/co-immunoprecipitation) experiments?
1. Cluttered WB Background-Antibody heavy chains (55kDa) and light chains (25kDa) interfere with the target band.
2. Excessive Incubation Time-Traditional Protein A/G requires overnight incubation, resulting in a lengthy experimental cycle.
3. Cumbersome Operation-Diverse reagents, complex configurations, and numerous steps.
4. Unstable Results-Large batch-to-batch variations and poor reproducibility.
AlpVHHs with its Nanoselector IP/Co-IP Kit series, brings you a brand-new solution, ending your troubles!
(1) Exgenous IP/Co-IP Kit:
High-performance nanobody magnetic beads with full tag coverage, including 21 common tags such as Flag, His, and GFP. Direct WB after IP, with no light or heavy chain interference throughout, resulting in cleaner IP/Co-IP data.
(2) Endogenous IP/Co-IP Kit:
Utilizes high-affinity nanobodies, specifically designed for Mouse/Rabbit IgG. Contains a dedicated secondary antibody to avoid light and heavy chains, perfectly solving the three major pain points of Protein A/G: serum IgG interference, affinity differences (e.g., low affinity of Protein A for mouse IgG1), and light and heavy chain contamination. IP/Co-IP results are purer and more reliable!
● One Box All You Need-Ready to Use Out of the Box
Magnetic beads + a complete set of buffers (lysis buffer/wash buffer/elution buffer) + negative/positive controls; no additional reagents required.
● Stable Entry -pM-Level Ultra-High Affinity
VHH nanobodies are covalently coupled to magnetic beads, ensuring stable performance and impressive loading capacity for highly efficient protein enrichment.
● Fast Entry-Rapid Incubation
Incubation at 4℃ requires only 0.5-1 hour for rapid capture, say goodbye to overnight waiting!
● Easy Operation-Magnetic Separation
No centrifugation required, simple separation with a magnetic rack saves time and effort, reduces protein loss, and improves recovery rate.
● Product Name: Mouse IgG & Rabbit IgG Nanoselector Magnetic beads IP Kit
● Product Specification: 20X Test
● Components
Buffer | Components | Volume | Storage Condition |
Mouse IgG&Rabbit IgG Nanoselector® Magnetic beads | 50% magnetic bead suspension, 10mM PBS pH 7.5, 0.01% proclin300 (Code:001-300-003) | 0.5mL | 4°C |
Lysis buffer | 10mM Tris-HCl pH7.5, 150mM NaCl, 0.5%NP40, 0.5mM EDTA | 30mL | 4°C |
Wash buffer | 10mM Tris-HCl pH7.5, 150mM NaCL, 0.05% NP40, 0.5mM EDTA | 60mL | 4°C |
Acidic elution buffer | 0.2M Glycine-HCl pH 2.5 | 10mL | 4°C |
Neutralization buffer | 1M Tris-HCl pH 10.4 | 1mL | 4°C |
2xSDS Sample buffer | 120mM Tris-HCL pH6.8, 20% Glycerol, 4% SDS, 0.04%bromophenol blue, 10% B-mercaptoethanol | 2mL | Room Temperature |
Mouse lgG Isotype Control | 1mg/ml Mouse IgG Isotype Control | 20uL | -20°C |
Rabbit IgG Isotype Control | 1mg/ml Rabbit IgG Isotype Control | 20uL | -20°C |
5XTBST | 0.1M Tris-HCl pH7.5, 2.5M NaCl, 0.5% Tween-20, 0.5% ProClin300 | 40mL | 4°C |
Anti-Mouse&Rabbit Heavy chain for IP, AlpSdAbs® VHH(HRP) | 1mg/ml VHH antibody (Code:001-190-005) Avoid light chains | 20uL | -20°C |
Anti-Mouse&RabbitLight chain for IP, AlpSdAbs® VHH(HRP) | 1mg/ml VHH antibody (Code: 001-180-005) Avoid heavy chains | 20uL | -20°C |
The Mouse IgG & Rabbit IgG Nanoselector® Magnetic Beads IP Kit is primarily used for immunoprecipitation (IP) or Co-IP experiments. This kit mainly consists of Mouse IgG & Rabbit IgG Nanoselector® magneticbeads and related reagents.
The Mouse IgG & Rabbit IgG Nanoselector® magnetic beads are composed of anti-Mouse IgG & Rabbit IgG nanobodies (VHH) covalently bound to magnetic agarose beads, primarily used for immunoprecipitation of Mouse IgG or Rabbit IgG antibodies. Compared to traditional protein A/G magnetic beads, Mouse IgG & Rabbit IgG Nanoselector® magnetic beads have higher affinity (KD < 1 nM), effectively binding more Mouse IgG and Rabbit IgG antibodies, making them a highly efficient research tool for binding Mouse IgG and Rabbit IgG.
● Related Products
Code | Description | Application |
001-101-003K | IP | |
025-101-003K | IP | |
001-300-003K | IP |
● Product Name: DYKDDDDK tag Nanoselector Magnetic beads IP Kit
● Product Specifications: 20X Test
● Components
Buffer | Components | Volume | Storage Condition |
DYKDDDDK tag Nanoselector® Magnetic beads | 50% magnetic bead suspension, 10mM PBS pH 7.5, 0.01% proclin300 (Code:016-101-003) | 500uL | 4°C |
Binding Control Magnetic beads | 50% magnetic bead suspension, 10mM PBS pH 7.5, 0.01% proclin300 (Code:100-100-200) | 500uL | 4°C |
Lysis buffer | 10mM Tris-HCl pH7.5, 150mM NaCL, 0.5% NP40, 0.5mM EDTA | 20mL | 4°C |
Wash buffer | 10mM Tris-HCl pH7.5, 150mM NaCl, 0.05% NP40, | 120mL | 4°C |
Acidic elution buffer | 0.2M Glycine-HCl pH 2.5 | 10mL | 4°C |
Neutralization buffer | 1M Tris-HCI pH 10.4 | 1mL | 4°C |
2xSDS Sample buffer | 120mM Tris-HCL pH6.8, 20% Glycerol, 4% SDS, 0.04%bromophenol blue, 10% B-mercaptoethanol | 2.5mL | Room Temperature |
5x TBST | 0.1M Tris-HCI pH7.5, 2.5M NaCI, 0.5% Tween-20, 0.5% ProClin300 | 60mL | 4°C |
Positive Reference (DYKDDDDK tag-mNeongreen)* | DYKDDDDK tag-mNeongreen in Lysis buffer | 1mL | 4°C |
The DYKDDDDK tag Nanoselector® Magnetic beads IP kit is primarily used for immunoprecipitation (IP) or Co-IP experiments. This kit mainly consists of DYKDDDDK tag Nanoselector® magnetic beads and related reagents.
The DYKDDDDK tag Nanoselector® magnetic beads are composed of an anti-DYKDDDDK tag protein nanobody (VHH) covalently bound to magnetic beads. It is used for immunoprecipitation of DYKDDDDK tag-fusion proteins from cell extracts of various organisms, including mammals, plants, bacteria, yeast, and insects, offering advantages such as high loading capacity and no light or heavy chain interference.
● Related Products
Code | Description | Application |
016-101-003K | IP | |
002-101-003K | IP | |
019-101-003K | IP | |
020-101-003K | IP | |
003-101-003K | IP | |
004-101-003K | IP |
The Nanoselector IP/Co-IP Kit leverages advanced nanobody technology combined with magnetic bead to deliver cleaner data and simplify your workflow. By effectively eliminating light and heavy chain interference, it removes a critical barrier in IP/Co-IP experiments, enabling more accurate detection and higher-confidence results—ultimately accelerating your research progress.
Backed by AlpVHHs’ expertise in nanobody engineering, the Nanoselector platform offers exceptional specificity, consistency, and ease of use. Whether you are working on protein interaction studies, biomarker discovery, or antibody validation, it empowers you to achieve reliable, reproducible outcomes with greater efficiency.
AlpVHHs Nanoselector - Your trusted expert for highly efficient Immunoprecipitation solution wthout light and heavy chain interference.
