In life science research, protein expression analysis, and biopharmaceutical development, DYKDDDDK-tag (Flag-tag) antibodies serve as widely used tools for tag detection. They are employed in various applications, including the verification of recombinant protein expression, immunoassays, protein-protein interaction studies, and purification analysis.
For a long time, the domestic research market has relied primarily on imported brands for high-quality Flag-tag antibodies. However, in recent years, factors such as shifts in global supply chains, the international trade environment, and uncertainties regarding delivery timelines have presented challenges-including reagent supply delays, extended procurement cycles, and rising costs-for some research projects.
Consequently, the market is actively seeking domestically produced alternatives that offer consistent quality, reliable performance, and timely delivery to ensure the continuity of scientific research.
As a biotechnology company specializing in the development of recombinant antibodies and nanobodies, Chengdu AlpVHHs Co., LTD. (AlpVHHs) leverages its proprietary high-throughput antibody screening platform, recombinant antibody engineering platform, and large-scale production system to provide high-performance antibody products to the global scientific research community.
Powered by its proprietary technology platforms, the Anti-DYKDDDDK Flag Tag Antibody developed by AlpVHHs offers:
● High lot-to-lot consistency
● High specificity and sensitivity
● Reliable supply chain
● Cost-effective alternative
As a domestic Chinese supplier of recombinant antibodies, AlpVHHs independently develops and manufactures all its products, offering a reliable alternative to imported products for research institutions, biopharmaceutical companies, and CRO.
Code | Description | Applications | Size |
016-303-001 | WB,ICC/IF,ELISA,IP,Flow Cyt | 100ug |
The DYKDDDDK peptide (Flag tag) is one of the most commonly used epitope tags in molecular biology. Due to its small size and minimal impact on protein structure and function, the Flag tag is widely fused to recombinant proteins for detection, characterization, purification, and functional studies.
Flag-tagged proteins can be efficiently detected through various experimental approaches, including:
● Western blotting (WB)
● Immunoprecipitation (IP)
● Immunofluorescence staining (ICC/IF)
● ELISA
● Flow cytometry
The Anti-DYKDDDDK Flag Tag Antibody enables researchers to conveniently monitor the expression, localization, and interaction of Flag-tagged proteins across different experimental workflows.
Anti-DYKDDDDK Flag Tag, AlpHcAbs® Mouse IgG2b Antibody is specifically designed for the detection of DYKDDDDK-tagged fusion proteins.
This antibody is generated using AlpVHHs' recombinant antibody engineering platform and is engineered to provide:
● Specific recognition of the DYKDDDDK Flag tag
● Minimal non-specific binding
● Excellent performance in multiple immunoassays
Validation experiments including Western blot (WB) and ELISA demonstrate strong and selective binding to DYKDDDDK-tagged proteins without detectable cross-reactivity with unrelated proteins.
Additionally, the Fc-engineered antibody design helps reduce Fc receptor-mediated background signals, improving assay reliability, especially in applications involving immune cells or complex biological samples.
● High lot-to-lot consistency
Recombinant antibody production ensures stable antibody quality between different production batches, supporting reproducible experimental results.
● Increased sensitivity and higher affinity
Optimized antibody affinity provides strong detection capability with low background signals, enabling reliable protein expression analysis.
● Animal-free production
The recombinant production process reduces dependence on animal immunization and supports consistent, scalable antibody manufacturing.
Secondary antibody：Anti-Mouse IgG(H+L), AlpHcAbs® Goat antibody(HRP)(001-401-005)(1:10000)
From antibody discovery to recombinant antibody manufacturing, AlpVHHs is committed to delivering innovative antibody products that accelerate life science research and biotechnology development.
With advanced recombinant antibody technologies and flexible supply capabilities, AlpVHHs supports researchers worldwide with reliable alternatives to traditional imported antibody products.
Explore AlpVHHs Anti-DYKDDDDK Flag Tag Antibody and experience a reliable domestic substitution solution for your research needs.
