Traditional IP processes are like a marathon, not only time-consuming and labor-intensive but also introducing numerous uncertainties. Critical Point Biotech has meticulously crafted two GFP IP solutions—GFP Nanoselector Magnetic beads (Code: 019-101-003) and the GFP Nanoselector Magnetic beads IP kit (Code: 019-101-003K)—aiming to completely liberate you from tedious and lengthy experimental procedures, redefining the speed and reliability of IP experiments with "ultimate simplification" and "ultimate efficiency"!
Core Disruption: What is the "5μL or 10min" Revolution?
Advantage 1: Ultra-high load capacity, high efficiency with minimal volume.
Only 5μL of magnetic beads are needed to efficiently complete a standard IP experiment. Less usage, higher load capacity, minimal volume for ultimate cost savings.
Fig 1. Tests with different amounts of GFP magnetic beads.
Advantage 2: Rapid Incubation, Quick and Stable Enrichment.
Only 10 minutes of incubation is needed to achieve highly efficient enrichment of GFP-tagged proteins. High speed and efficiency eliminate the long wait times of traditional methods (hours or even overnight), significantly shortening the experimental cycle. Combined with HRP direct-labeling antibodies, results can be obtained in just one day.
Fig 2. Tests of GFP magnetic beads at different incubation times
Advantage 3: Flexible elution conditions without compromising performance
Extremely high stability, with GFP Nanoselector remaining stable even at 70℃. Furthermore, besides operating in physiologically concentrated salt solutions, GFP Nanoselector can tolerate harsh environments such as high salt and acid/alkali conditions, exhibiting rock-solid protein binding stability.
Fig 3. Tests of GFP magnetic beads under different elution condition
Strength Verified by Data
We firmly believe that accurate and rigorous data is the most powerful proof of product performance.
Fig 4. Effect of Ser residue mutation on SKIP31 phosphorylation (Yi H, et al., 2025)
Testing Conditions: Immunoprecipitation was performed using GFP Nanoselector Magnetic beads (Code: 019-101-003).
Results: A single, clear target protein band was obtained in plant samples with high enrichment efficiency and excellent specificity. An article entitled "BIK1-mediated phosphorylation and SCFSKIP31-mediated ubiquitination coordinately control CNGC3 homeostasis and activity to fine-tune plant immunity" was published in the journal *Molecular Plant* (IF=24.1/Q1).
What Makes Our Magnetic Beads Stand Out?
Unveiling the Core Technology Behind Superior Performance
Nanoselector® magnetic beads are composed of nano-tagged antibodies (VHH) covalently bound to magnetic agarose beads. They are used for immunoprecipitation of tagged fusion proteins from cell extracts of various organisms, including mammals, plants, bacteria, yeast, and insects, offering advantages such as high loading capacity and no interference from light or heavy chains.
Looking for ultimate flexibility and cost control? Choose GFP Nanoselector Magnetic beads (Code: 019-101-003), with core magnetic beads that can be freely combined with your preferred buffer system, making it the ideal choice for your experiments. Seeking maximum efficiency and out-of-the-box convenience? Choose the GFP Nanoselector Magnetic beads IP kit (Code: 019-101-003K). This kit provides a complete set of components, building a one-stop IP workflow. It includes a positive/negative control system, effectively reducing variables, significantly shortening time, and ensuring experimental success rates, helping you easily achieve "out-of-the-box, one-time success." The road to scientific exploration is full of challenges, and a reliable tool is key to success. AlpVHHs' GFP Nanoselector series is more than just a product; it's a pure, efficient, and worry-free experimental solution we provide for you.
Code | Description | Application |
019-101-003 | IP,CHIP,MS,Purification | |
019-101-003K | IP |
Learn More
Visit www.alpvhhs.com to explore Nanoselector Magnetic Beads and discover how nanobody technology can elevate your IP, Co-IP, and proteomics workflows.
