Service
Consult
Contact
Telephone:
+86 138 8067 6215
Address:
Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130
Email:
Platform
Phage Display Technology Platform
Phage display is a technology based on displaying foreign peptides, antibodies or proteins on the surface of phages. It can screen out specific antibodies or proteins with high throughput, so it is widely used in new drug development, antibody screening, vaccine development and other fields. The technology was first reported by George P. Smith and others in 1985, and was applied in the field of antibody engineering by John McCafferty and Gregory Winter in the early 1990s. After continuous improvement and perfection, it has become one of the important tools in the field of modern biomedical research. In 2018, two pioneers in phage display research, American scientist George P. Smith and British scientist Gregory P. Winter, won the Nobel Prize in Chemistry.
Phage display principle
Phage display technology is to insert exogenous peptides, antibodies and other fragments into the structural genes of phages. It is common to express them by fusion with PIII or PVIII. The molecules displayed on the surface of phages still maintain biological activity. To select the desired molecules from the phage library, they must go through "screening". Simply put, the process is that the peptide library or protein library displayed on the surface of the phage can specifically recognize and bind to the target antigen. After a sufficient period of incubation, the free phage that is weakly bound to the antigen or not bound can be washed away with a washing solution. The specifically bound target phage is then eluted, infected with E. coli and amplified to obtain the next round of daughter phage library. After 2 to 3 rounds of "adsorption-elution-amplification" enrichment process, the proportion of phages that can specifically bind to the antigen is gradually increased. Finally, a peptide or protein that can recognize the target molecule is obtained and can be used in subsequent experiments.
Advantages of AlpVHHs:
1. Based on "zero background" vector technology, the library insertion rate reaches 100%
2. Self-developed super-competent state, one electroporation can make the library capacity reach 109
3. Flexible and changeable screening strategies and screening methods, making the screening success rate reach more than 90%
4. 800+ project development experience, with extentive experience.
