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Telephone:
+86 138 8067 6215
Address:
Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130
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Platform
Yeast Display Technology Platform
Yeast surface display (YSD) is a powerful protein engineering technology and an important high-throughput screening platform in protein engineering. Yeast cells have eukaryotic post-translational modification mechanisms that can help target proteins fold correctly and can be used to display various eukaryotic proteins, including antibodies, receptors, enzymes, and antigenic peptides.
Technical Principle
The AlpVHHs yeast surface display platform uses the "α-lectin" display system to insert the nanobody gene sequence and the expression tag HA tag gene sequence into the display plasmid vector of the protein scaffold-Aga2 gene. The vector uses nutritional markers to maintain selective growth in yeast, and galactose is added to the culture medium to induce yeast to display on the surface. The nanobody fusion protein is secreted and anchored on the yeast cell wall. Combined with magnetic beads and flow sorting technology for multiple rounds of screening, high-affinity and high-stability clones can be screened.
Advantages of yeast display:
1. Eukaryotic expression system can ensure the true folding of proteins
2. Uniform growth of yeast reduces screening bias
3. Screening can be refined through flow sorting, such as affinity screening, blocker, epitope screening, etc.
4. Compatible with NGS, convenient for obtaining a large amount of post-selection information
Advantages of AlpVHHs:
AlpVHHs is the earliest company in China to engage in the research and development of yeast display technology platform, with the world's leading yeast display platform.
1. Improved super yeast competence, one electrotransformation can reach 108
2. Improved strains and vectors can make the expression efficiency of the library reach more than 90%
3. 500+ project experience, with rich experience
