Nano-DT
DT-3C is a commonly used endocytosis detection reagent, but its binding domain is protein G, which will bind to IgG in serum and cause interference. Secondly, the affinity of protein G to different subtypes of mouse and human IgG is different, which will interfere with the result judgment. Nano-DT replaces the protein G binding domain in DT-3C with a high-affinity, high-specificity nanobody that does not bind to bovine IgG.
Nano-DT is an efficient antibody endocytosis detection tool that can be used as a high-throμghput detection tool for pre-drμg evaluation of ADC-related antibodies.
Overview:
● Nanobody-based detection system enhancing traditional DT-3C assays
● Provides rapid and reliable internalization readout
Sensitive Functional Internalization Assessment
● VHH nano-secondary conjugated diphtheria toxin fragments
● High-sensitivity functional readout of antibody internalization
● Suitable for low-expression targets and early-stage ADC feasibility studies
Provides reliable validation of internalization capability during high-throughput screening.
Technical Advantages:
● Improved signal-to-noise ratio
● Supports diverse antibody types and cell lines
● Ready-to-use reagents to reduce experimental complexity
All Products
| Code | Description | Applications | Size |
| 001-101-020 | Anti-Mouse IgG, AlpSdAbs® VHH(DT) | Internalization Test | 100ug |
| 023-101-020 | Anti-Human IgG, AlpSdAbs® VHH(DT) | Internalization Test | 100ug |
