Nano-pH-sensitive probe is a new generation of nanobodies (<15KD) coupled to pH-dependent fluorescent probes for endocytosis detection launched by AlpVHHs. pH-sensitive dyes have no fluorescence excitation outside the cell, but emit bright red or green fluorescence in some acidic environments inside the cell (including phagosomes). Unlike traditional labeling methods using amino or thiol-reactive markers, Nano-pH-sensitive probes, which are site-specifically coupled to pH-dependent fluorescent probes by specific nanobodies, are very stable and compatible with BSA and other stable proteins.
Nano-pH-sensitive probe is a fast and economical antibody endocytosis detection reagent.