Nano-pH-sensitive probe
Nano-pH-sensitive probe is a new generation of nanobodies (<15KD) coupled to pH-dependent fluorescent probes for endocytosis detection launched by AlpVHHs. pH-sensitive dyes have no fluorescence excitation outside the cell, but emit bright red or green fluorescence in some acidic environments inside the cell (including phagosomes). Unlike traditional labeling methods using amino or thiol-reactive markers, Nano-pH-sensitive probes, which are site-specifically coupled to pH-dependent fluorescent probes by specific nanobodies, are very stable and compatible with BSA and other stable proteins.
Nano-pH-sensitive probe is a fast and economical antibody endocytosis detection reagent.
Overview:
● Fluoresces in acidic endosomal/lysosomal compartments for real-time monitoring
● Available dyes: Deep Red 630, pH-Red 600
Quantitative Evaluation and Real-Time Imaging
● VHH nano-secondary conjugated pH-sensitive fluorescent probes
● Fluoresces in acidic endosomal/lysosomal compartments
● Enables live-cell imaging and flow cytometry analysis
● Quantitative assessment of antibody internalization efficiency
Perfect for mechanistic studies, trafficking analysis, and high-content imaging during antibody screening.
Technical Advantages:
● Track ADC internalization in live cells
● High specificity reduces background
● Compatible with confocal microscopy and high-throughput assays
All Products
| Code | Description | Applications | Size |
| 001-101-012 | Anti-Mouse IgG, AlpSdAbs® VHH(pH-Red 600) | Internalization Test | 100ug |
| 001-101-013 | Anti-Mouse IgG, AlpSdAbs® VHH(pHAb) | Internalization Test | 100ug |
| 001-101-014 | Anti-Mouse IgG, AlpSdAbs® VHH(Deep Red630) | Internalization Test | 100ug |
| 023-101-012 | Anti-Human IgG, AlpSdAbs® VHH(pH-Red 600) | Internalization Test | 100ug |
| 023-101-013 | Anti-Human IgG, AlpSdAbs® VHH(pHAb) | Internalization Test | 100ug |
| 023-101-014 | Anti-Human IgG, AlpSdAbs® VHH(Deep Red630) | Internalization Test | 100ug |
| 023-102-012 | Anti-Human IgG Fc, AlpSdAbs® VHH(pH-Red 600 ×8) | Internalization Test | 100ug |
| 023-102-014 | Anti-Human IgG Fc, AlpSdAbs® VHH(Deep Red630 ×8) | Internalization Test | 100ug |
| APK021 | pH630 Deep Red TFP Ester, Amine Reactive | ICC/IF,Flow Cyt | 100ug |
| APK022 | pH630 Deep Red maleimide, Thiol Reactive | ICC/IF,Flow Cyt | 100ug |
| APK023 | pHRed600 TFP Ester, Amine Reactive | ICC/IF | 100ug |
| APK024 | pHRed600 maleimide, Thiol Reactive | ICC/IF | 100ug |
