Code: 023-101-014
Size: 100ug
Applications: Internalization Test
Specificity: Human IgG
Conjugate: Deep Red630 (Ex: 640nm, Em: 655nm), 2 moles Deep Red630 per mole VHH
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Anti-Human IgG, AlpSdAbs® VHH(Deep Red630) is a human IgG detection antibody designed for studying the internalization of antibodies. This monovalent, recombinant single-domain VHH antibody derived from alpaca heavy chain variable regions is directly conjugated to Deep Red630. Based on immunoelectrophoresis and/or ELISA, it reacts with human IgG specifically. It is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.
It consists of VHH nano-secondary conjugated pH-sensitive fluorescent probes that fluoresce in acidic endosomal/lysosomal compartments, enable live-cell imaging and flow cytometry analysis, and provide quantitative assessment of antibody internalization efficiency. Perfect for mechanistic studies, trafficking analysis, and high-content imaging during antibody screening.
Immunogen | Recombinant human IgG |
Host | Alpaca pacous |
Isotype | VHH domain of alpaca IgG2b/2c |
Conjugate | Deep Red630 (Ex: 640nm, Em: 655nm), 2 moles Deep Red630 per mole VHH |
Specificity | Human IgG |
Cross-Reactivity | Recognizes human IgG specifically, and reacts with cynomolgus IgG. No cross-reactivity with rabbit, mouse, rat, goat IgG |
Purity | Recombinant Expression and Affinity purified |
Concentration | 0.5 mg/ml |
Formulation | Liquid, 10mM PBS (pH 7.4), 50% Glycerol |
Storage | Store at –20 °C (Avoid freeze / thaw cycles), Protect from light |
VHH nano-secondary conjugated pH-sensitive fluorescent probes
Fluoresces in acidic endosomal/lysosomal compartments
Enables live-cell imaging and flow cytometry analysis
Quantitative assessment of antibody internalization efficiency
Perfect for mechanistic studies, trafficking analysis, and high-content imaging during antibody screening
Track ADC internalization in live cells
High specificity reduces background
Compatible with confocal microscopy and high-throughput assays
Want to explore more pH-sensitive fluorescent tools? Click here to learn more about Nano-pH-sensitive probe series
Antibody Internalization Test: 4ug per 10ug test antibody
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Deep Red630 has pH-sensitive fluorescence excitation/emission spectra of 610/640 nm, and its fluorescence emission increases in intensity with increasing acidity. This increase is particularly dramatic in the range pH 4.5–9, as commonly seen within endocytic vesicles. Deep Red630 is essentially dark in the extracellular environment; however, upon internalization, it elicits a bright fluorescent signal in the acidic environment of the endosomes.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small (<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Request COA or Quality Certificate for detailed batch information
Contact Technical Support for dilution recommendations or application guidance
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