Code: 016-101-003
Size: 0.5 mL(20 rxns) / 5 mL(200 rxns)
Applications: IP,CHIP,MS,Purification
Specificity: DYKDDDDK tag
Conjugate: Magnetic beads
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
Product Detail
Our DYKDDDDK tag Nanoselector Magnetic Beads are designed for rapid, high-efficiency capture and purification of DYKDDDDK-tagged fusion proteins. Based on recombinant anti-DYKDDDDK VHH nanobodies, these magnetic beads provide high affinity, large binding capacity, and consistent results even under harsh conditions. Ideal for IP, Co-IP Kit applications, ChIP, mass spectrometry (MS), and enzyme activity measurements, they simplify protein purification workflows while minimizing non-specific background. Compatible with physiological and stringent buffers, these beads offer flexibility for diverse experimental setups. Explore our recombinant tag antibody collection to complement your research.
Description:
DYKDDDDK tag Nanoselector Magnetic beads have been specifically designed to bind DYKDDDDK tag-fusion proteins. DYKDDDDK tag Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. DYKDDDDK tag Nanoselector Magnetic beads are ideal tools to isolate or purify DYKDDDDK tag-fusion proteins fast and efficiently.
High Affinity: Single-domain nanobody (VHH) ligand ensures strong, specific binding to DYKDDDDK-tagged proteins.
Large Binding Capacity: 10 µL slurry binds ~20 µg of recombinant DYKDDDDK-fusion protein.
Consistent & Reproducible: Covalent VHH attachment prevents leakage of light and heavy chains.
Fast & Efficient: Short incubation (5–30 min) accelerates immunoprecipitation workflows.
Versatile Compatibility: Works under physiological and high-stringency conditions.
No Antibody Chain Interference: Single-domain format avoids heavy/light chain contamination.
| Traditional Anti-FLAG Antibody IP | DYKDDDDK Nanoselector® Magnetic Beads | |
|---|---|---|
| Capture ligand | Conventional IgG (heavy + light chain), often polyclonal or hybridoma-derived | Single-domain VHH nanobody, recombinantly produced |
| Incubation time | Typically 1–4 hours, often overnight | 5–30 minutes |
| Elution contamination | Heavy/light chains can co-elute and overlap with target protein on SDS-PAGE / MS | Covalent VHH attachment — no antibody chain leakage into your sample |
| Lot-to-lot consistency | Variable, especially with polyclonal or hybridoma sources | Recombinant production eliminates batch-to-batch variation |
| Binding affinity | Antibody-dependent, often nM range | Engineered for high affinity (Kd down to 1 pM for the Nanoselector platform) |
| Buffer compatibility | Affinity can drop under high-salt / high-stringency wash | Stable under both physiological and high-stringency conditions |
| Best for downstream MS | Risk of antibody-derived peptide interference | Clean elution supports mass spectrometry workflows |
This makes DYKDDDDK Nanoselector Magnetic Beads a direct, drop-in upgrade for labs currently running FLAG-tag IP/Co-IP with conventional monoclonal or polyclonal antibodies — same workflow, shorter hands-on time, cleaner downstream data.
| Code | Specification | Applications | Binding Capacity | Storage |
|---|---|---|---|---|
| 016-101-003 | 0.5 mL (20 rxns) / 5 mL (200 rxns) | IP, Co-IP Kit, ChIP, MS, Protein Purification | 10 µL slurry binds ~20 µg DYKDDDDK-fusion protein | Store at 4°C, stable 1 year |
Ligand: Anti-DYKDDDDK tag single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm
Reactivity: The DYKDDDDK tag Nanoselector recognizes the DYKDDDDK-tag sequence of the fusion protein.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20µg of recombinant DYKDDDDK- fusion protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
The DYKDDDDK peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, DYKDDDDK tag is unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
DYKDDDDK tag Nanoselector Magnetic beads has been specifically designed to bind DYKDDDDK tag. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. DYKDDDDK tag Nanoselector Magnetic beads thus features high affinity and superior capacity for DYKDDDDK tag fusion proteins while showing negligible non-specific background. DYKDDDDK tag Nanoselector Magnetic beads is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
Protein Purification of DYKDDDDK-tag fusion proteins
Immunoprecipitation (IP / Co-IP Kit) for target protein enrichment
ChIP / RIP Analysis for chromatin or RNA-binding protein studies
Mass Spectrometry (MS) sample preparation
Enzyme Activity Assays for functional studies
Fluorescent Protein Detection when combined with downstream assays
| Stock status | Made to order — not held in regular inventory |
| Standard lead time | Approx. 4 weeks (~1 month) from order confirmation |
| Custom volumes / bulk orders | Available — contact us to discuss pricing and timeline |
| Minimum Order Quantity (MOQ) | Contact us for MOQ details |
Planning a project with a fixed start date? Because this product is manufactured to order, we recommend placing your order at least 4–6 weeks ahead of when you need it in hand. Contact our team early and we'll confirm the current lead time and help you plan around it.
DYKDDDDK Nanoselector Magnetic Beads are manufactured under AlpVHHs’ ISO 9001-certified quality management system, with a 5-stage quality control process applied to every production batch:
IQC (Incoming Quality Control) → IPQC (In-Process QC) → FQC (Final QC)→ OQC (Outgoing QC) → governed by an overarching QMS (Quality Management System).
The recombinant VHH ligand used in this product is produced via animal-free recombinant expression, ensuring high lot-to-lot consistency.
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Request Certificate of Analysis (COA) →
Traditional anti-FLAG IP relies on a conventional IgG antibody (with heavy and light chains) typically captured on Protein A/G beads. DYKDDDDK Nanoselector beads use a single-domain VHH nanobody covalently attached directly to the magnetic bead — there's no antibody chain to leak into your eluate, no Protein A/G cross-reactivity, and a much shorter incubation time (5–30 min vs. 1+ hours).
No — because the VHH ligand is covalently attached and there are no antibody heavy or light chains to co-elute, the eluate is cleaner for SDS-PAGE and MS compared to traditional antibody-based IP.
10 µL of bead slurry binds approximately 20 10 µg of recombinant DYKDDDDK-tagged fusion protein.
Shipped at ambient temperature. Upon receipt, store at 4°C (do not freeze). Stable for 1 year under recommended storage conditions (50% slurry in PBS with 20% ethanol).
This product is manufactured to order rather than held in inventory. Standard lead time is approximately 4 weeks (~1 month) from order confirmation. If your project has a hard deadline, contact us as early as possible so we can confirm current capacity and help you plan your timeline.
Yes — see the related DYKDDDDK tag Nanoselector® Magnetic Beads IP Kit for a complete workflow solution.
Request a Quote: Contact us for bulk orders or custom volumes.
Download Datasheet: Access detailed protocols and technical specifications.
Technical Support: Our specialists provide guidance for IP, Co-IP Kit setups, and protein purification optimization.
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Immunoprecipitation-DYKDDDDK tag Nanoselector Magnetic beads
