Smart Ligands
As a professional Nanobody (VHH) Discovery Platform and nanobody ligands supplier, AlpVHHs® provides Smart Ligands® — recombinant biotinylated VHH nanobody ligands engineered for reliable site-directed immobilization of antibodies and target proteins in SPR, BLI, ELISA, purification, and biosensor applications. Compared with conventional Protein A/G capture systems, Smart Ligands® deliver superior orientation control, reduced steric hindrance, enhanced binding accessibility, and highly reproducible kinetic analysis for antibody discovery and characterization workflows.
Smart Ligands® are recombinant biotinylated VHHs designed to capture non-biotinylated immunoglobulins or target proteins specifically onto streptavidin/avidin-coated surfaces. These recombinant affinity ligands enable oriented antibody immobilization while preserving antigen-binding activity and improving assay reproducibility.
Unlike conventional immobilization methods that often generate random antibody orientation, Smart Ligands® selectively recognize Fc, Fab, light chain, or tag epitopes, enabling:
Site-directed antibody capture
Improved antigen accessibility
More accurate kinetic analysis
Reduced nonspecific background
Better regeneration stability
Enhanced signal consistency
Smart Ligands® are widely used in:
SPR (Surface Plasmon Resonance)
BLI (Biolayer Interferometry)
ELISA
Biosensor assays
Antibody affinity analysis
Epitope binning
Antibody screening
Protein purification workflows
All Products
| Code | Description | Applications | Size |
| 001-101-004 | Anti-Mouse IgG(H+L), AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 001-102-004 | Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 001-103-004 | Anti-Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 001-104-004 | Anti-Mouse IgG2b(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 001-105-004 | Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 001-106-004 | Anti-Mouse IgG kappa, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 001-107-004 | Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 001-108-004 | Anti-Mouse IgG1(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 003-101-004 | Anti-HA tag, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 004-101-004 | Anti-His tag, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 006-101-004 | Anti-SUMO tag, AlpSdAbs® VHH(Biotin) | WB,ELISA,IP | 100ug |
| 010-101-004 | Anti-GST, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 012-101-004 | Anti-Halo tag, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100uL |
| 013-101-004 | Anti-mNeongreen, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 014-101-004 | Anti-turboGFP, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 015-101-004 | Anti-MBP, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 017-103-004 | Anti-TagFP, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 019-101-004 | Anti-GFP, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 020-101-004 | Anti-RFP, AlpSdAbs® VHH(Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 023-101-004 | Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(Biotin) | WB,ELISA,Purification,BLI,SPR | 100ug |
| 023-102-004 | Anti-Human kappa, AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 023-103-004 | Anti-Human lambda, AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 023-104-004 | Anti-Human IgG(Fab Fragment specific), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 023-105-004 | Anti-Human IgG(CH1 Fragment specific), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 023-106-004 | Anti-Human IgG(H+L), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP | 100ug |
| 023-107-004 | Anti-Human IgE, AlpSdAbs® VHH(Biotin) | ELISA,IP,Purification,BLI,SPR | 100ug |
| 023-108-004 | Anti-Human IgD, AlpSdAbs® VHH(Biotin) | ELISA,IP,Purification,BLI,SPR | 100ug |
| 023-109-004 | Anti-Human IgA, AlpSdAbs® VHH(Biotin) | ELISA,IP,Purification,BLI,SPR | 100ug |
| 023-110-004 | Anti-Human IgM, AlpSdAbs® VHH(Biotin) | ELISA,IP,Purification,BLI,SPR | 100ug |
| 024-101-004 | Anti-Chicken IgY, AlpSdAbs® VHH (Biotin) | ELISA,Purification,BLI,SPR | 100ug |
| 025-101-004 | Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) | WB,ELISA,Purification,BLI,SPR | 100ug |
| 025-102-004 | Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 025-103-004 | Anti-Rabbit IgG(Fab Fragment specific), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 025-104-004 | Anti-Rabbit IgG kappa, AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 054-101-004 | Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) | WB,ELISA,Purification,BLI,SPR | 100ug |
| 054-102-004 | Anti-Goat IgG(H+L), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 061-101-004 | Anti-mGold, AlpSdAbs® VHH(Biotin) | ELISA,IP | 100ug |
| 064-101-004 | Anti-V5 tag, AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 071-101-004 | Anti-Rat IgG(H+L), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 071-102-004 | Anti-Rat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 071-103-004 | Anti-Rat IgG(Fab Fragment specific), AlpSdAbs® VHH(Biotin) | WB,ELISA,IP,Purification,BLI,SPR | 100ug |
| 082-101-004 | Anti-StayGold, AlpSdAbs® VHH(Biotin) | WB,ELISA,IP | 100ug |
| Features | Smart Ligands® (VHH Ligands) | Protein A/G |
|---|---|---|
| Immobilization Orientation | Site-directed | Random |
| Molecular Size | Ultra-small VHH (<15 kDa) | Large protein |
| Steric Hindrance | Minimal | Higher |
| Antigen Accessibility | Excellent | Variable |
| Binding Specificity | Fc/Fab/subclass-specific | Broad Fc binding |
| Regeneration Stability | High | Moderate |
| Kinetic Reproducibility | Excellent | Variable |
| Crude Sample Compatibility | Yes | Limited |
| Recombinant Production | Yes | Usually not |
| Batch Consistency | High | Variable |
Because VHH nanobodies are significantly smaller than conventional antibodies or Protein A/G molecules, Smart Ligands® minimize steric hindrance and preserve the native orientation of captured antibodies. This is especially critical in:
SPR kinetic analysis
BLI affinity measurements
Epitope mapping
ADC characterization
High-throughput antibody screening
Smart Ligands® enable oriented antibody capture by selectively recognizing defined Fc/Fab regions or tags, helping preserve antigen-binding sites for more reliable interaction analysis.
Selected recombinant VHH ligands exhibit dissociation constants down to the picomolar range, supporting highly stable antibody immobilization and sensitive biosensor detection.
The reduced steric hindrance and defined orientation improve:
Association rate measurements (ka)
Dissociation rate measurements (kd)
KD determination accuracy
Epitope accessibility
making Smart Ligands® ideal for antibody characterization workflows.
Smart Ligands® maintain stable capture performance through repeated regeneration cycles, improving sensor reusability and assay reproducibility.
Smart Ligands® can efficiently capture antibodies directly from crude supernatants or partially purified samples, reducing sample preparation complexity.
All Smart Ligands® are produced through recombinant expression systems to ensure:
High lot-to-lot consistency
Stable long-term supply
Reproducible experimental results
Animal-free manufacturing
Biotinylated VHH ligands are immobilized onto streptavidin-coated biosensor surfaces.
Target antibodies are specifically captured through Fc, Fab, subclass, or tag recognition.
Antigen interactions are analyzed using:
SPR
BLI
Biosensor platforms
Captured antibodies can be removed while maintaining Smart Ligand® activity for repeated assay cycles.
Smart Ligands® improve antibody orientation and kinetic reproducibility for:
Affinity analysis
KD determination
Epitope binning
Competition assays
Ideal for:
High-throughput antibody screening
Real-time binding analysis
Antibody ranking
Biosimilar characterization
Smart Ligands® support highly specific antibody capture with low background and improved signal consistency.
Widely used in:
Hybridoma screening
Nanobody discovery
Recombinant antibody screening
Therapeutic antibody development
High-affinity VHH ligands provide selective purification of Fc-, Fab-, or tag-containing proteins.
| Parameter | Smart Ligands® |
|---|---|
| Ligand Type | Recombinant biotinylated VHH |
| Immobilization Method | Streptavidin/Avidin capture |
| Affinity | Up to pM range |
| Production | Recombinant expression |
| Regeneration Compatibility | Excellent |
| Sample Type | Purified or crude samples |
| Applications | SPR, BLI, ELISA, purification |
| Batch Consistency | High |
| Steric Hindrance | Minimal |
AlpVHHs® specializes in recombinant nanobody and single-domain antibody technologies with extensive experience in:
Yeast display platforms
Phage display platforms
Recombinant VHH engineering
Antibody discovery
Nanobody conjugation
Biosensor assay development
Our recombinant nanobody products are widely used in antibody discovery, therapeutic development, biosensor analysis, and high-throughput screening applications.
Smart Ligands® are recombinant biotinylated VHH nanobody ligands designed for oriented antibody immobilization and biosensor-based interaction analysis.
Because VHHs are ultra-small and highly stable, they minimize steric hindrance and improve antigen accessibility, resulting in more accurate kinetic measurements.
Yes. In many SPR and BLI workflows, Smart Ligands® provide superior orientation control, better reproducibility, and improved regeneration stability compared with Protein A/G systems.
Yes. Smart Ligands® are optimized for affinity and kinetic studies including ka, kd, and KD determination.
Yes. Smart Ligands® are compatible with crude supernatants and partially purified antibody samples.
Yes. All Smart Ligands® are recombinant VHH-based affinity ligands produced through animal-free recombinant expression systems.
Looking for recombinant VHH ligands for SPR, BLI, ELISA, or antibody immobilization workflows?
AlpVHHs® provides:
Custom nanobody ligand development
Species-specific VHH ligands
Fc/Fab-selective affinity ligands
Biosensor assay optimization
Bulk supply for CRO and biopharma applications
Contact our technical team for product recommendations, datasheets, or custom ligand solutions.
