TiterPlus® Adjuvant Rabbit

Details and Advantages

Code: 600-000-003

Specification: 1mL / 10mL

Applications: Immunization

Reactivity:

Conjugate:

Advantages:

Product Description Manual MSDS FAQ Citations

Product Detail

Description:

TiterPlus® Adjuvant Rabbit is a novel water-soluble adjuvant specifically formulated for rabbit immunization, particularly suitable for preparing rabbit monoclonal/polyclonal antibodies. Compared with conventional Freund's adjuvant, it offers numerous advantages, including fewer immunization injections, lower antigen dosage, faster antibody production, higher antibody titers, higher antibody affinity, preservation of the antigen's natural conformation, and ease of use.

Storage Conditions:

Store at 4ºC. (Do not freeze!)

Instructions for Use:

1. Dilute the antigen to twice the final concentration with physiological saline (prepare 100 μl per injection).

Note: Recommended antigen dosages are: ① 20-50 μg per injection for subunit protein antigens with weak immunogenicity; ② 5-10 μg per injection for inactivated whole viruses or bacteria and virus-like particle antigens with strong immunogenicity.

2. Thoroughly mix the adjuvant. Under aseptic conditions, take the required amount (100 μl per injection) and quickly mix it with the antigen at a volume ratio of 1:1.

Note: Precipitation from this adjuvant is normal. The faster the mixing with the antigen, the better.

3. Immunize rabbits via intramuscular injection in the hind leg (video). Inject 200 μl per rabbit.

Note:

①Precipitation from this adjuvant after mixing with the antigen is normal. Thoroughly mix before drawing into the syringe and inject as soon as possible after drawing into the syringe

② Subcutaneous or intradermal injection can also be used for immunization according to experimental practice.

Precautions and Disclaimers:

1. Transport at room temperature and store at 4°C. Do not freeze, as freezing will affect the effectiveness of the adjuvant.

2. The adjuvant contains precipitate, which is an effective component of the adjuvant. Please ensure thorough mixing before use.

There are two methods for mixing: ① Shake well; ② Mix by blowing with a pipette tip.

3. Please mix and use immediately. Take the adjuvant and antigen to the animal facility and mix thoroughly. Immunize the animal within 10 minutes of mixing. Do not allow too long an interval, as the adjuvant contains precipitate, which will settle and cause uneven distribution of the active ingredient, affecting the immunization results.

This product is for scientific research use by professionals only and must not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals. For your safety and health, please wear a lab coat and disposable gloves when handling.

FAQ

Citations