Service
Consult
Contact
Telephone:
+86 138 8067 6215
Address:
Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130
Email:
Technical Service
Nano Antibody Labeling Platform
Nanobodies (Nbs) are versatile molecular tools that are used in many areas of biological sciences, such as immunofluorescence microscopy and structural biology. In order to meet detection needs, nanobodies often need to be labeled.
A large amount of data statistics show that more than half of the interactions are mediated by CDR3, CDR2 accounts for 20%, and CDR1 accounts for 13%. Surprisingly, 16% of the interactions are established by residues in the nanobody framework (non-CDR), which are concentrated in four structurally different regions, and non-CDR interactions are mainly mediated by residues in the framework 2 region, and the residues involved are mostly charged residues. Nanobodies have a relatively delicate structure, and conventional active amino-based labeling can easily inactivate antibodies. At the same time, this type of labeling method is difficult to control the labeling ratio. Critical Point specializes in nanobody development and can provide fixed-point nanobody labeling services, which can not only maintain antibody activity but also clarify the labeling ratio.
I. Service Content
1. Biotin labeling
2. DBCO labeling
3. Horseradish peroxidase (HRP), alkaline phosphatase (AP/ALP)
4. Fluorescein labeling
FITC, Rhodamine B (RBITC)
Alexa Fluor® 350, 405, 488, 555, 594, 647, 680, 750, etc.
DyLight® series 350, 405, 488, 555, 594, 647, 680, 750, etc.
iFlour® series 350, 405, 488, 555, 594, 647, 680, 750, etc.
Biotium CF® series: 350, 405, 488, 555, 594, 647, 680, 750, etc.
Cy dyes: Cy3, Cy3B, Cy5, Cy5.5, Cy7
ATTO series: 390, 425, 488, 555, 594, 647, 680, 750, etc.
Cy dyes: Cy3, Cy3B, Cy5, Cy5.5, Cy7
Fluorescent proteins: Phycoerythrin (PE), Allophycocyanin (APC);
Fluorescent tandem labeling:
PE-Cy fluorescein series: PE-Cy5, PE-Cy5.5, PE-Cy7;
APC-Cy fluorescein series: APC-Cy5.5, APC-Cy7;
PerCP-Cy fluorescein series: PerCP-Cy5.5;
5. DNA labeling
6. Toxin labeling
MMAE, MMAF, DXD, PBD, etc.
7. Agarose and magnetic beads
II. Service Process
1. The customer submits a request for commissioned marking and specifies the marking requirements, agrees with the company on specific experimental items, quantity and costs, and both parties sign the "Commissioned Marking Preparation Contract".
2. The customer provides samples to be marked, and our company arranges marking services in accordance with the contract requirements and completes the marking commission within the specified time.
3. After the marking is completed, our company will package according to the customer's requirements, mail them to the customer free of charge at low temperature, and provide a marking report at the same time.
Ⅲ. Cases
AlpVHHs uses optimized site-specific coupling technology to ensure that antibodies are efficiently labeled with biotin while ensuring that antibody affinity is not affected, facilitating subsequent testing and application.
AlpVHHs uses optimized fluorescent dye labeling technology to test and optimize the coupling with a variety of fluorescent dyes to determine the best antibody: dye molecule ratio, thereby producing a conjugate with the highest specific signal intensity. At the same time, multiple technologies are used to optimize bioconjugation chemistry and strict purification to remove free dyes and antibodies, thereby producing higher quality products.
