Anti-CD16a, AlpHcAbs® Human antibody

Details and Advantages

Code: 300-516-001

Specification:

Applications: ELISA,Flow Cyt

Reactivity: Human/Cynomolgus

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-CD16a, AlpHcAbs® Human antibody is designed for detecting human CD16a specifically. Anti-CD16a, AlpHcAbs® Human antibody is recombinant VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc. Based on ELISA, Anti-CD16a, AlpHcAbs® Human antibody reacts with human CD16a, and has no reactivity with CD16b.

Immunogen: Recombinant human CD16a
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: Human CD16a
Cross-Reactivity: Cross-reactivity with cynomolgus CD16a
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

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