Human IgG1(L234A, L235A), kappa Isotype Control

Details and Advantages

Code: 400-101-025

Specification: 1mg / 20mg

Applications: Isotype Control

Reactivity:

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Human IgG1(L234A, L235A), kappa Isotype Control is designed as a negative control in Human IgG1 antibody(with the mutation L234A, L235A) staining applications. Human IgG1(L234A, L235A), kappa Isotype Control belongs to the same immunoglobulin class and subclass as the Human IgG1 antibody(with the mutation L234A, L235A) which is used in the experiment but lack affinity to the protein of interest. Human IgG1(L234A, L235A), kappa Isotype Control makes experiments credible,

Isotype: Human IgG1(kappa)
Conjugate: Unconjugated
Specificity: HEL(hen egg-white lysozyme)
Purity: Human cell production, Recombinant Expression and Affinity purified, > 95%(HPLC)
Concentration: 1mg/ml
Endotoxin: < 0.2 EU/mg(LAL gel clotting)
Formation: Liquid, 10mM PBS (pH 7.5)
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)

Background:
Antibodies bind to a specific protein or antigen, but sometimes an antibody can bind to other proteins or cellular components in a non-specific manner. Experimental controls are critical in determining if the antibodies used in your experiment have any off-target binding capabilities. Off-target or non-specific binding by antibodies lead to false positive results, making it difficult to determine background staining from antigen-specific staining. Therefore, it is important to always include experimental controls to ensure reliable results.

Application Notes:

Negative control for Human IgG1(kappa) antibody(with the mutation L234A, L235A)
Using at a dilution as the test antibody which is used in the experiment.

FAQ

Citations