Description
The Isotyping Kit for Mouse Monoclonal Antibody (APK001) is a powerful research tool designed for qualitative isotype determination of mouse immunoglobulins. This kit enables accurate identification of mouse immunoglobulin isotypes, including IgG1, IgG2a, IgG2b, IgG3, IgM, and kappa from hybridoma cell culture supernatant or purified antibodies by Enzyme Linked Immunosorbent Assay (ELISA). This kit consists of Goat Fc fused nanobody against mouse antibody isotypes, with higher specificity and sensitivity than most available products.
Materials provided:

Note:
• Sufficient reagents are provided to perform over 5000 Clones by ELISA.
• All the Detection Antibodies should be diluted 1:10000 in dilution buffer before use.
• All the Detection Antibodies should be protected from prolonged exposure to light.
Benifits
Guaranteed 100% Accuracy
No background
Monoclonal Ab
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Specificity
The Isotyping Kit for Mouse Monoclonal Antibody is highly specific, capable of identifying every subtype and isotype of antibodies existing in Hybridoma cell supernatant or purified forms. All the isotype specific antibodies used in the kit are well characterised, and have no cross-reactivity with other isotypes.
Note:
• Samples were coated on polystyrene multiwell plates under 37℃ for 2 hours, and detected by Isotyping Kit for Mouse Monoclonal Antibody.
• All the Detection Antibodies were diluted 1:10000 in dilution buffer before use.
Storage/Stability
Store at –20℃, stable for one year from date of receipt. Avoid repeated freeze-thaw cycles.