Anti-Rabbit IgG(Fab Fragment specific), AlpSdAbs® VHH(iFluor594 ×4)

Details and Advantages

Code: 025-103-008

Specification: 100ug / 1mg

Applications: WB,ELISA,ICC/IF,Flow Cyt

Reactivity: Rabbit IgG(Fab Fragment specific)

Conjugate: iFluor594

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Rabbit IgG(Fab Fragment specific), AlpSdAbs® VHH(iFluor594 ×4) is designed for detecting Fab region of rabbit IgG specifically. Anti-Rabbit IgG(Fab Fragment specific), AlpSdAbs® VHH(iFluor594 ×4) is based on monovalent, recombinant single domain antibody to rabbit IgG(Fab Fragment specific) coupled to iFluor594. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG(Fab Fragment specific), AlpSdAbs® VHH(iFluor594 ×4) reacts with the Fab region of rabbit IgG selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant Rabbit IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor594(Ex: 592nm, Em: 614nm)
Specificity: Fab region of Rabbit IgG
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles) , Protect from light.

Background:
Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance

Flow Cyt: 1:200-1:2000
ICC/IF： 1:200-1:2000
ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
Super-resolution microscopy

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations