Anti-Sheep IgG(H+L), AlpSdAbs® VHH(iFluor488 ×8)

Details and Advantages

Code: 055-101-007

Specification: 100ug / 1mg

Applications: WB,ELISA,ICC/IF,Flow Cyt

Reactivity: Sheep IgG(H+L)

Conjugate: iFluor488

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti- Sheep IgG(H+L), AlpSdAbs® VHH(iFluor488 ×8) is designed for detecting sheep IgG specifically. Anti- Sheep IgG(H+L), AlpSdAbs® VHH(iFluor488 ×8) is based on monovalent, recombinant single domain antibody to sheep IgG coupled to iFluor488. Based on immunoelectrophoresis and/or ELISA, Anti- Sheep IgG(H+L), AlpSdAbs® VHH(iFluor488 ×8) reacts with the Sheep IgG selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat IgG.

Immunogen: Sheep IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor488(Ex: 495nm, Em: 519nm)
Specificity: Sheep IgG
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance

ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
Flow Cyt: 1:100-1:1000
ICC/IF： 1:100-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations

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