Mouse IgG&Rabbit IgG Nanoselector® Magnetic beads IP Kit
Code: 001-300-003K
Specification: 20X Test
Applications: IP
Reactivity: Mouse&Rabbit
Conjugate:
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinarybinding,even under harsh conditions
High affinity and Short incubation (5-30min)
Product Detail
Product Detail
Description:
Mouse IgG&Rabbit IgG Nanoselector® Magnetic beads IP Kit is designed for IP(immunoprecipitation) or Co-IP experiment.This kit consist of Mouse IgG&Rabbit IgG Nanoselector® Magnetic beads and related supporting reagents.
The Mouse IgG&Rabbit IgG Nanoselector® Magnetic beads consists of anti-Mouse IgG&Rabbit IgG Nanobody (VHH), which is covalently bound to magnetic beads. Mouse IgG&Rabbit IgG Nanoselector® Magnetic beads is used to immunoprecipitate Mouse IgG or Rabbit IgG antibody. Compared with protein A/G, Mouse IgG&Rabbit IgG nanoselector Magnetic beads has much higher affinity than protein A/G, affinity KD<1nM. Mouse IgG&Rabbit IgG Nanoselector Magnetic beads is effective to pull down mouse IgG or rabbit IgG, and 10 µL slurry bind about 100 µg of mouse IgG or rabbit IgG.
Properties
Ligand | Anti-Mouse IgG&Rabbit IgG single domain antibody fragment (VHH, Nanobody) |
Reactivity | Specifically binds to Mouse IgG or Rabbit IgG |
Binding capacity | 100 µg of Mouse IgG or Rabbit IgG per 10 µL bead slurry |
Bead size | ~ 40 µm |
Storage buffer | 10mM PBS (pH 7.5), 0.01% proclin300 |
Storage conditions | Upon receipt store at +4°C. Do not freeze! |
Stability | Stable for 1 year upon receipt. |
Shipment | Shipped at low temperature(2-8℃) |
Main Components of The Kit
Name | Composition | Quantity | Storage |
Mouse IgG&Rabbit IgG Nanoselector® Magnetic beads | 50% Magnetic beads slurry, 10mM PBS pH 7.5, 0.01% proclin300(Code: 001-300-003) | 0.5 mL | 4 ℃ |
Lysis buffer | 10mM Tris-HCl pH7.5,150mM NaCl, 0.5%NP40, 0.5mM EDTA | 30 mL | 4 ℃ |
Wash buffer | 10mM Tris-HCl pH7.5, 150mM NaCl, 0.05% NP40, 0.5mM EDTA | 60 mL | 4 ℃ |
Acidic elution buffer | 0.2M Glycine-HCl pH 2.5 | 10 mL | 4 ℃ |
Neutralization buffer | 1M Tris-HCl pH 10.4 | 1 mL | 4 ℃ |
2xSDS Sample buffer | 120mM Tris-HCL pH6.8, 20% Glycerol, 4% SDS, 0.04% bromophenol blue, 10% β-mercaptoethanol | 2 mL | Room Temperature |
Mouse IgG Isotype Control | 1mg/ml Mouse IgG Isotype Control | 20 uL | -20 ℃ |
Rabbit IgG Isotype Control | 1mg/ml Rabbit IgG Isotype Control | 20 uL | -20 ℃ |
5x TBST | 0.1M Tris-HCl pH7.5, 2.5M NaCl, 0.5% Tween-20, 0.5% ProClin300 | 40 mL | 4 ℃ |
Anti-Mouse&Rabbit Heavy chain for IP, AlpSdAbs® VHH(HRP) | 1mg/ml VHH antibody (Code: 001-190-005) | 20 uL | -20 ℃ |
Anti-Mouse&Rabbit Light chain for IP, AlpSdAbs® VHH(HRP) | 1mg/ml VHH antibody (Code: 001-180-005) | 20 uL | -20 ℃ |
Note:
1. Please ensure that the tube cover is tightly closed after each use to avoid affecting the performance of the reagent.
2. Each component needs to be stored under appropriate temperature conditions.
3. When selecting secondary antibodies, please pay attention to Anti-Mouse &Rabbit Heavy Chain for IP, AlpSdAbs® VHH (HRP) secondary antibody only recognizes the heavy chain of the primary antibody, and when WB exposure it can avoid band interference near the light chain(approximately 25kDa). Anti-Mouse&Rabbit Light chain for IP, AlpSdAbs® VHH(HRP) secondary antibody only recognizes the light chain of the primary antibody, and when WB exposure it can avoid band interference near the heavy chain(approximately 55kDa). If there is no need to consider the interference of light and heavy chains, either of the above two antibodies can be used.
Product Application:
Immunoprecipitation(IP), Co-immunoprecipitation(CO-IP), Enzyme activity detection, Mass spectrometry analysis (MS), etc.
Note: Please refer to the instruction manual for usage instructions.
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