DYKDDDDK tag Nanoselector Magnetic beads
Code: 016-101-003
Specification: 0.5 mL(20 rxns) / 5 mL(200 rxns)
Applications: IP,CHIP,MS,Purification
Reactivity: DYKDDDDK tag
Conjugate: Magnetic beads
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
Product Detail
Product Detail
Description:
DYKDDDDK tag Nanoselector Magnetic beads have been specifically designed to bind DYKDDDDK tag-fusion proteins. DYKDDDDK tag Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. DYKDDDDK tag Nanoselector Magnetic beads are ideal tools to isolate or purify DYKDDDDK tag-fusion proteins fast and efficiently.
Ligand: Anti-DYKDDDDK tag single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm
Reactivity: The DYKDDDDK tag Nanoselector recognizes the DYKDDDDK-tag sequence of the fusion protein.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20µg of recombinant DYKDDDDK- fusion protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
The DYKDDDDK peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, DYKDDDDK tag is unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
DYKDDDDK tag Nanoselector Magnetic beads has been specifically designed to bind DYKDDDDK tag. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. DYKDDDDK tag Nanoselector Magnetic beads thus features high affinity and superior capacity for DYKDDDDK tag fusion proteins while showing negligible non-specific background. DYKDDDDK tag Nanoselector Magnetic beads is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
Applications:
Protein purification
Immunoprecipitation (IP)/Co-IP
Mass spectrometry (MS)
Enzyme activity measurements
ChIP/ RIP analysis
FAQ
Citations
Related Products
Consult
Contact
Telephone:
+86 138 8067 6215
Address:
Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130
Email:
Immunoprecipitation-DYKDDDDK tag Nanoselector Magnetic beads
