Halo Nanoselector Magnetic beads

Details and Advantages

Code: 012-101-003

Specification: 0.5 mL(20 rxns) / 1 mL(40 rxns)

Applications: IP,CHIP,MS,Purification

Reactivity: Halo tag

Conjugate: Magnetic beads

Advantages:

Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)

Product Description Manual MSDS FAQ Citations

Product Detail

Description
Halo Nanoselector Magnetic beads have been specifically designed to bind Halo-fusion proteins. Halo Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. Halo Nanoselector Magnetic beads are ideal tools to isolate or purify Halo-fusion proteins fast and efficiently.

Ligand: Anti-Halo single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for Halo fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant Halo protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300

Background
The protein tag (HaloTag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (HaloTag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Halo-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the Halo Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

Applications:

Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis

FAQ

Citations