mGold Nanoselector Magnetic beads

Details and Advantages

Code: 061-101-003

Specification: 0.5mL(20 rxns) / 1mL(40 rxns)

Applications: IP,CHIP,MS,Purification

Reactivity: Magnetic beads

Conjugate: mGold

Advantages:

Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)

Product Description Manual MSDS FAQ Citations

Product Detail

Description
mGold Nanoselector Magnetic beads have been specifically designed to bind mGold-fusion proteins. mGold Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. mGold Nanoselector Magnetic beads are ideal tools to isolate or purify mGold-fusion proteins fast and efficiently.

Ligand: Anti-mGold single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for mGold, mGold2s, mGold2t fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mGold protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300

Background
mGold is a yellow fluorescent protein (YFPs), and its variants mGold2s and mGold2t are the brightest and most photostable monomeric yellow fluorescent proteins, breaking the traditional trade-off between photostability and brightness and achieving simultaneous enhancement of both properties. Compared to widely used YFPs such as mVenus and mCitrine, their photostability has increased by 25 times, with minimal changes to other biophysical properties.
mGold-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mGold Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

Performance

Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis

FAQ

Citations