mNeongreen Nanoselector Magnetic beads

Details and Advantages

Code: 013-101-003

Specification: 0.5 mL(20 rxns) / 1 mL(40 rxns)

Applications: IP,CHIP,MS,Purification

Reactivity: mNeongreen

Conjugate: Magnetic beads

Advantages:

Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
mNeongreen Nanoselector Magnetic beads have been specifically designed to bind mNeongreen-fusion proteins. mNeongreen Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. mNeongreen Nanoselector Magnetic beads are ideal tools to isolate or purify mNeongreen-fusion proteins fast and efficiently.

Ligand: Anti-mNeongreen single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for mNeongreen fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mNeongreen protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300

Background:
mNeongreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single molecule super-resolution imaging and is an excellent fluorescence resonance energy transfer(FRET) acceptor for the newest cyan fluorescent proteins. mNeongreen-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mNeongreen Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

Applications:

Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis

FAQ

Citations