SUMO tag Nanoselector Magnetic beads
Code: 006-101-003
Specification: 0.5mL(20 rxns) / 1mL(40 rxns)
Applications: IP,CHIP,MS,Purification
Reactivity: SUMO tag
Conjugate: Magnetic beads
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
Product Detail
Product Detail
Description:
SUMO tag Nanoselector Magnetic beads have been specifically designed to bind SUMO tag-fusion proteins. SUMO tag Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. SUMO tag Nanoselector Magnetic beads are ideal tools to isolate or purify SUMO tag-fusion proteins fast and efficiently.
Ligand: Anti-SUMO tag single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for SUMO tag fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant SUMO tag fusion protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300
Background:
SUMO tag is short for Small Ubiquitin-like Modifier, the 100 amino acid sequence, which is necessary for regulation of protein transport and is important for controlling transcription for eukaryotic cells. Sumo tag is most frequently used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein. SUMO proteins are similar to ubiquitin in their folded structure but possess only about 20% homology to the amino acid sequence of ubiquitin. The number of SUMO genes varies among eukaryotes. SUMO proteins are conjugated to substrate proteins in a manner similar to ubiquitin. In addition, SUMO attachment can be removed by SUMO-specific isopeptidases just as UCHs and UBPs remove ubiquitin from substrate proteins.
SUMO tag-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the SUMO tag Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
Performance
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
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