Rabbit IgG Nanoselector® Magnetic beads IP Kit

Details and Advantages

Code: 025-101-003K

Specification: 20X Test

Applications: IP

Reactivity: Rabbit

Conjugate:

Advantages:

Consistent and reproducible results
No heavy & light antibody chains
Extraordinarybinding,even under harsh conditions
High affinity and Short incubation (5-30min)

Product Description Manual MSDS FAQ Citations

Product Detail

Description:

Rabbit IgG Nanoselector® Magnetic beads IP Kit is designed for IP(immunoprecipitation) or Co-IP experiment.This kit consist of Rabbit IgG Nanoselector® Magnetic beads and related supporting reagents.

The Rabbit IgG Nanoselector® Magnetic beads consists of an anti-Rabbit IgG Nanobody (VHH), which is covalently bound to magnetic beads. Rabbit IgG Nanoselector® Magnetic beads is used to immunoprecipitate Rabbit IgG antibody. Compared with protein A/G, Rabbit IgG nanoselector Magnetic beads has much higher affinity than protein A/G, affinity KD<1nM. Rabbit IgG Nanoselector Magnetic beads is effective to pull down rabbit IgG, and 10 µL slurry bind about 100 µg of rabbit IgG.

Properties

Ligand	Anti-Rabbit IgG single domain antibody fragment (VHH, Nanobody)
Reactivity	Specifically binds to Rabbit IgG
Binding capacity	100 µg of Rabbit IgG per 10 µL bead slurry
Bead size	~ 40 µm
Storage buffer	10mM PBS (pH 7.5), 0.01% proclin300
Storage conditions	Upon receipt store at +4°C. Do not freeze!
Stability	Stable for 1 year upon receipt.
Shipment	Shipped at low temperature(2-8℃)

Main Components of The Kit

Name	Composition	Quantity	Storage
Rabbit IgG Nanoselector^® Magnetic beads	50% Magnetic beads slurry, 10mM PBS pH 7.5, 0.01% proclin300 (Code: 025-101-003)	0.5 mL	4 ℃
Lysis buffer	10mM Tris-HCl pH7.5,150mM NaCl, 0.5%NP40, 0.5mM EDTA	30 mL	4 ℃
Wash buffer	10mM Tris-HCl pH7.5, 150mM NaCl, 0.05% NP40, 0.5mM EDTA	60 mL	4 ℃
Acidic elution buffer	0.2M Glycine-HCl pH 2.5	10 mL	4 ℃
Neutralization buffer	1M Tris-HCl pH 10.4	1 mL	4 ℃
2xSDS Sample buffer	120mM Tris-HCL pH6.8, 20% Glycerol, 4% SDS, 0.04% bromophenol blue, 10% β-mercaptoethanol	2 mL	Room Temperature
Rabbit IgG Isotype Control	1mg/ml Rabbit IgG Isotype Control	20 ul	-20 ℃
5x TBST	0.1M Tris-HCl pH7.5, 2.5M NaCl, 0.5% Tween-20, 0.5% ProClin300	40 mL	4 ℃
Anti-Mouse&Rabbit Heavy chain for IP, AlpSdAbs® VHH(HRP)	1mg/ml VHH antibody(Code: 001-190-005)	20 uL	-20 ℃
Anti-Mouse&Rabbit Light chain for IP, AlpSdAbs® VHH(HRP)	1mg/ml VHH antibody (Code: 001-180-005)	20 uL	-20 ℃

*Note:

1. Please ensure that the tube cover is tightly closed after each use to avoid affecting the performance of the reagent.

2. Each component needs to be stored under appropriate temperature conditions.

3. When selecting secondary antibodies, please pay attention to Anti-Mouse &Rabbit Heavy Chain for IP, AlpSdAbs® VHH (HRP) secondary antibody only recognizes the heavy chain of the primary antibody, and when WB exposure it can avoid band interference near the light chain(approximately 25kDa). Anti-Mouse&Rabbit Light chain for IP, AlpSdAbs® VHH(HRP) secondary antibody only recognizes the light chain of the primary antibody, and when WB exposure it can avoid band interference near the heavy chain(approximately 55kDa). If there is no need to consider the interference of light and heavy chains, either of the above two antibodies can be used.

Product Application:

Immunoprecipitation(IP), Co-immunoprecipitation(CO-IP), Enzyme activity detection, Mass spectrometry analysis (MS), etc.

Note: Please refer to the instruction manual for usage instructions.

FAQ

Citations