RFP Nanoselector® Magnetic beads IP Kit

Details and Advantages

Code: 020-101-003K

Specification: 20X Test

Applications: IP

Reactivity: RFP

Conjugate:

Advantages:

Consistent and reproducible results
No heavy & light antibody chains
Extraordinarybinding,even under harsh conditions
High affinity and Short incubation (5-30min)

Product Description Manual MSDS FAQ Citations

Product Detail

Description:

RFP Nanoselector® Magnetic beads IP Kit is designed for IP(immunoprecipitation) or Co-IP experiment. This kit consist of RFP Nanoselector® Magnetic beads and related supporting reagents.

The RFP Nanoselector® Magnetic beads consists of an anti-RFP Nanobody (VHH), which is covalently bound to magnetic beads. RFP Nanoselector® Magnetic beads is used to immunoprecipitate RFP-fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. The RFP Nanoselector® Magnetic beads has great advantages such as high load capacity and no light or heavy chain interference.

Properties

Ligand	Anti-RFP single domain antibody fragment (VHH, Nanobody)
Reactivity	Recognizes mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives.
Binding capacity	20 µg of recombinant RFP-fusion protein per 10 µL bead slurry
Bead size	~ 40 µm
Storage buffer	10mM PBS (pH 7.5), 0.01% proclin300
Storage conditions	Upon receipt store at +4°C. Do not freeze!
Stability	Stable for 1 year upon receipt.
Shipment	Shipped at low temperature(2-8℃)

Main Components of The Kit

Name	Composition	Quantity	Storage
RFP Nanoselector^® Magnetic beads	50% Magnetic beads slurry, 10mM PBS pH 7.5, 0.01% proclin300(Code: 020-101-003)	500 uL	4 ℃
Binding Control Magnetic beads	50% Magnetic beads slurry, 10mM PBS pH 7.5, 0.01% proclin300(Code: 100-100-200)	500 uL	4 ℃
Lysis buffer	10mM Tris-HCl pH7.5,150mM NaCl, 0.5%NP40, 0.5mM EDTA	20 mL	4 ℃
Wash buffer	10mM Tris-HCl pH7.5, 150mM NaCl, 0.05% NP40, 0.5mM EDTA	120 mL	4 ℃
Acidic elution buffer	0.2M Glycine-HCl pH 2.5	10 mL	4 ℃
Neutralization buffer	1M Tris-HCl pH 10.4	1 mL	4 ℃
2xSDS Sample buffer	120mM Tris-HCL pH6.8, 20% Glycerol, 4% SDS, 0.04% bromophenol blue, 10% β-mercaptoethanol	2.5 mL	Room Temperature
5x TBST	0.1M Tris-HCl pH7.5, 2.5M NaCl, 0.5% Tween-20, 0.5% ProClin300	60 mL	4 ℃
Positive Control (RFP-his)*	RFP-his in Lysis buffer	1 mL	4 ℃

*Note:

1. When using a Positive Control, please add 500ul of Positive Control/25 ul magnetic beads to each IP reaction.

2. Please ensure that the tube cover is tightly closed after each use to avoid affecting the performance of the reagent.

3. Each component needs to be stored under appropriate temperature conditions.

Product Application:

Immunoprecipitation(IP), Co-immunoprecipitation(CO-IP), Enzyme activity detection, Mass spectrometry analysis (MS), etc.

Note: Please refer to the instruction manual for usage instructions.

FAQ

Citations