SpyCatcher003 Magnetic beads

Details and Advantages

Code: 086-102-003

Specification: 0.5 mL(20 rxns) / 1 mL(40 rxns)

Applications: IP,CHIP,MS,Purification

Reactivity: SpyTag003

Conjugate: Magnetic beads

Advantages:

Consistent and reproducible results
Extraordinary binding, even under harsh conditions
Short Incubation (5-30 min)

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
SpyCatcher003 Magnetic beads have been specifically designed to bind SpyTag003-fusion proteins. SpyCatcher003 Magnetic beads are based on Spycatcher003 covalently coupled to the surface of magnetic beads, and they are used to detect and isolate SpyTag003-fusion proteins efficiently.

Ligand: SpyCatcher003
Bead size: ~ 40 µm
Reactivity: Highly selective for SpyTag003-fusion proteins.
Binding Capacity: High binding capacity, 10 µL slurry bind about 10-20µg of SpyTag003-fusion protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300.

Background:
The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag). Upon recognition, the two form a covalent isopeptide bond between the side chains of a lysine in SpyCatcher and an aspartate in SpyTag. This technology has been used, among other applications, to create covalently stabilized multi-protein complexes, for modular vaccine production, and to label proteins. The SpyTag system is versatile as the tag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins; similarly, SpyCatcher can be fused to reporter proteins such as GFP, and to epitope or purification tags.

SpyCatcher003-SpyTag003 system is a variant of SpyCatcher-SpyTag system, the 003 mutations is able to improve both the noncovalent association rate and the rate of reaction following this noncovalent docking.

Performance

Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis

FAQ

Citations