StayGold/mBaojin Nanoselector Magnetic beads

Details and Advantages

Code: 082-101-003

Specification: 0.5 mL(20 rxns) / 1 mL(40 rxns)

Applications: IP,CHIP,MS,Purification

Reactivity: StayGold/mBaojin

Conjugate: Magnetic beads

Advantages:

Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
StayGold/mBaojin Nanoselector Magnetic beads have been specifically designed to bind StayGold/mBaojin-fusion proteins. StayGold/mBaojin Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. StayGold/mBaojin Nanoselector Magnetic beads are ideal tools to isolate or purify StayGold/mBaojin-fusion proteins fast and efficiently.

Ligand: Anti-StayGold single domain antibody fragment (VHH, Nanobody)

Bead size: ~ 40 µm

Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.

Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant StayGold.

Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.

Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol

Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging. For biochemical analysis including mass spectrometry and enzyme activity measurements these StayGold-fusion proteins and their interacting factors need to be isolated fast and efficiently by immunoprecipitation using the StayGold Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

Applications:

Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis

FAQ

Citations