Code: 023-102-012
Size: 100ug / 1mg
Applications: Internalization Test
Specificity: Human IgG
Conjugate: pH-sensitive Red 600
Advantages:
Product Detail
Description:
Anti-Human IgG Fc, AlpSdAbs® VHH(pH-Red 600 ×8) is designed for studying on the internalization of antibodies. Anti-Human IgG Fc, AlpSdAbs® VHH(pH-Red 600 ×8) is based on recombinant single domain antibody to human IgG Fc coupled to pH-Red 600. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG Fc, AlpSdAbs® VHH(pH-Red 600 ×8) reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG. Anti-Human IgG Fc, AlpSdAbs® VHH(pH-Red 600 ×8) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.
Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: pH-sensitive Red 600(Ex: 576nm, Em: 597nm), 2 moles Red 600 per mole VHH
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.5mg/ml
Formation: Liquid, 10mM PBS (pH 7.4), 50% Glycerol
Storage: Store at -20 °C(Avoid freeze / thaw cycles), Protect from light
Background:
pH-Red 600 has pH-sensitive fluorescence excitation/emission spectra of 576/597 nm, and its fluorescence emission increases in intensity with increasing acidity. This increase is particularly dramatic in the range pH 4.5–9, as commonly seen within endocytic vesicles. pH-Red 600 is essentially dark in the extracellular environment; however, upon internalization, it elicits a bright fluorescent signal in the acidic environment of the endosomes.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
Antibody Internalization Test: 4ug per 10ug test antibody
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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