Code: 001-101-012
Size: 100ug
Applications: Internalization Test
Specificity: Mouse IgG
Conjugate: pH-sensitive Red 600(Ex: 576nm, Em: 597nm), 2 moles Red 600 per mole VHH
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Anti-Mouse IgG, AlpSdAbs® VHH(pH-Red 600) is based on a recombinant single domain antibody coupled to pH-Red 600, specifically designed for studying on the internalization of antibodies.
validated by immunoelectrophoresis and/or ELISA, this single domain antibodies is an an effective detection tools that reacts with mouse IgG for the evaluation of antibody potency prior to antibody-drug conjugate(ADCs).
Track ADC internalization in live cells
High specificity reduces background
Compatible with confocal microscopy and high-throughput assays
Immunogen | Mouse IgG |
Host | Alpaca pacous |
Isotype | VHH domain of alpaca IgG2b/2c |
Conjugate | pH-sensitive Red 600(Ex: 576nm, Em: 597nm), 2 moles Red 600 per mole VHH |
Specificity | Mouse IgG |
Cross-Reactivity | No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG |
Purity | Recombinant Expression and Affinity purified |
Concentration | 1mg/ml |
Formation | Liquid, 10mM PBS (pH 7.4), 50% Glycerol |
Storage | Store at –20 °C(Avoid freeze / thaw cycles), Protect from light |
Anti-Mouse IgG, AlpSdAbs® VHH (pH-Red 600) is a next-generation nano pH-sensitive probe developed by AlpVHHs. It consists of a recombinant single-domain antibody (VHH, <15 kDa) site-specifically conjugated to the pH-Red 600 fluorescent dye. The probe exhibits excellent tissue penetration and high stability while retaining high-affinity and specific binding to mouse IgG. It is ideally suited for mechanistic studies, intracellular trafficking analysis, and high-content imaging during antibody screening and characterization.
pH-Red 600 has pH-sensitive fluorescence excitation/emission spectra of 576/597 nm, and its fluorescence emission increases in intensity with increasing acidity. This increase is particularly dramatic in the range pH 4.5–9, as commonly seen within endocytic vesicles. pH-Red 600 is essentially dark in the extracellular environment; however, upon internalization, it emits a bright fluorescent signal in the acidic endosomal environment.
Antibody Internalization Test: 2ug per 10ug test antibody
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Anti-Mouse IgG, AlpSdAbs® VHH (pH-Red 600) is a next-generation pH-sensitive nanobody probe specifically designed for tracking antibody endocytosis and ADC internalization in live cells. It offers high specificity, bright endosomal signals, and excellent stability.
Contact our technical support team today to request a quote, detailed protocols, or samples.
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