Code: APK021
Size: 100ug / 1mg
Applications: ICC/IF,Flow Cyt
Specificity:
Conjugate:
Advantages:
Product Detail
pH630 Deep Red TFP Ester is an amine-reactive, pH-sensitive fluorescent dye designed for precise antibody internalization assays, endocytosis, and phagocytosis studies. This pH-activated fluorescent dye readily reacts with primary amines on proteins, cells, or viruses to form stable covalent conjugates that remain non-fluorescent at neutral pH and become fluorescent only in acidic environments.
Unlike conventional fluorescent dyes, pH630 Deep Red remains non-fluorescent at neutral pH, significantly reducing background signal. Upon internalization into acidic compartments such as late endosomes and lysosomes, the dye undergoes pH-dependent fluorescence activation, making it an ideal tool for lysosome tracking and endocytosis assays with a high signal-to-noise ratio.
Maximum Excitation: ~640 nm
Emission Values: ~655 nm
Detection: Standard Cy5 filter sets
Reactive Group: TFP ester (amine-reactive)
pKa: ~5
Molecular Weight: ~1300 Da
Storage conditions: -20℃
pH-Activated Fluorescence
Selectively fluoresces in acidic environments, enabling precise detection of intracellular localization.
Low Background & Superior Specificity
Virtually no fluorescence at neutral pH → exceptional assay accuracy, reduced optimization steps, and dramatically cleaner results compared to non-pH-sensitive dyes.
Amine-Reactive Labeling
TFP Ester enables efficient, stable conjugation to primary amines on proteins, cells, or viruses.
Optimized for Internalization Assays
Ideal for antibody internalization studies, receptor-mediated endocytosis, and phagocytosis assays.
Cy5-Compatible Detection
Easily integrated into existing workflows for flow cytometry, fluorescence microscopy, and high-content screening (HCS).
pH630 Deep Red provides superior discrimination between intracellular and extracellular substances. With a pK_a ≈ 5, the dye remains dark at physiological pH and is selectively activated only upon entry into acidic late endosomes and lysosomes. This high pH sensitivity enables a clear, unambiguous distinction between cell-surface-bound and internalized biomolecules, ensuring high-fidelity results in internalization and phagocytosis assays.
This unique pH-responsive property enables the development of rapid, wash-free assays, ensuring high certainty of results for tracking antibody internalization, endocytosis, and phagocytosis pathways. These features make pH630 Deep Red particularly highly valuable for Antibody-Drug Conjugate (ADC) development, where precise monitoring of intracellular delivery is critical for therapeutic efficacy.
Antibody internalization assays
Lysosome and endosome tracking
Phagocytosis assays
Live cell imaging
Flow cytometry analysis
High-content screening (HCS)
Lower pKa for more precise late-endosomal/lysosomal activation
True no-wash, no-quencher protocols that save time and reduce variability
Superior performance in ADC internalization and delivery validation
Contact us today for protocol details, free sample requests, technical support, or bulk pricing.
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