Code: 023-101-103
Size: 100ug / 1mg
Applications: Internalization Test
Specificity: Human IgG(Fcγ Fragment specific)
Conjugate: GGFG-DXD(2 moles GGFG-DXD per mole VHH)
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Anti-Human IgG Fc, AlpSdAbs® VHH (GGFG-DXD ×4) is a monoclonal recombinant single-domain antibody (sdAb) directed against the human IgG Fc region and conjugated to GGFG-DXD. Specifically engineered for antibody internalization assays, this VHH has been validated via immunoelectrophoresis and/or ELISA to react exclusively with the Fc fragment of the human IgG heavy chain, exhibiting no cross-reactivity with Fab fragments. Consequently, it serves as a potent analytical tool for assessing antibody potency during the early stages of antibody-drug conjugate (ADC) development
Immunogen | Recombinant Fc region of human IgG |
Host | Alpaca pacous |
Isotype | VHH domain of alpaca IgG2b/2c |
Conjugate | GGFG-DXD(2 moles GGFG-DXD per mole VHH) |
Specificity | Human IgG(Fcγ fragment specific), including IgG1, IgG2, IgG3, IgG4 and their mutations(LALA or YTE) |
Cross-Reactivity | Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG |
Purity | Recombinant Expression and Affinity purified |
Concentration | 0.5mg/ml |
Formation | Liquid, 10mM PBS (pH 7.4) |
Storage | Store at –20 °C(Avoid freeze / thaw cycles) |
VHHs are single-domain antibodies (sdAbs) derived from the variable regions of camelid heavy-chain-only antibodies. Characterized by an exceptionally low molecular weight ($<15$ kDa) compared to conventional antibody fragments, VHHs exhibit superior tissue penetration, extreme stability, and high environmental tolerance compared to conventional antibody fragments. Consequently, these nanobodies represent indispensable tools across various domains, including basic research, diagnostics, and therapeutic development.
Antibody Internalization Test: 2ug per 10ug test antibody(molar ratio=2:1).
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
This Anti-Human IgG Fc AlpSdAbs® VHH (GGFG-DXD ×4) is specifically designed for early-stage ADC internalization evaluation. It offers high specificity and excellent stability.
Contact technical supports to get detailed experimental protocols and a quote.
Or browse our Nano-secondary antibody-Drug conjugates to know more.
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