Code: 023-101-101
Size: 100ug
Applications: Internalization Test
Specificity: Human IgG(Fcγ Fragment specific)
Conjugate: VcMMAE
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Description:
Anti-Human IgG Fc, AlpSdAbs® VHH(VcMMAE ×4) is designed for studying on the internalization of antibodies. Anti-Human IgG Fc, AlpSdAbs® VHH(VcMMAE ×4) is based on monoclonal, recombinant single domain antibody to human IgG Fc coupled to VcMMAE. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG Fc, AlpSdAbs® VHH(VcMMAE ×4) reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG. Anti-Human IgG Fc, AlpSdAbs® VHH(VcMMAE ×4) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.
Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: VcMMAE(2 moles VcMMAE per mole VHH)
Specificity: Human IgG(Fcγ fragment specific), including IgG1, IgG2, IgG3, IgG4 and their mutations(LALA or YTE)
Cross-Reactivity: Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.5mg/ml
Formation: Liquid, 10mM PBS (pH 7.4)
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
Antibody Internalization Test: 2ug per 10ug test antibody(molar ratio=2:1).
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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Contact
Telephone:
+86 17882311198
Address:
Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130
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A titer ELISA of different mutation-Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH
The plate was coated with different amounts of IgG or its variants. 1:5000 dilution of Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH was used as the detection antibody.
A titer ELISA of Anti-Human IgG, AlpSdAbs® VHH(VcMMAE)
The plate was coated with different amounts of different isotope of Human IgG. 1:10000 dilution of Anti-Human IgG, AlpSdAbs® VHH(VcMMAE) was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG(004-202-005) as the secondary antibody.
