DogCatcher Magnetic beads
Code: 086-103-003
Specification: 0.5 mL(20 rxns) / 1 mL(40 rxns)
Applications: IP,CHIP,MS,Purification
Reactivity: DogTag
Conjugate: Magnetic beads
Advantages:
Consistent and reproducible results
Extraordinary binding, even under harsh conditions
Short Incubation (5-30 min)
Product Detail
Product Detail
Description:
DogCatcher Magnetic beads have been specifically designed to bind DogTag-fusion proteins. DogCatcher Magnetic beads are based on Dogcatcher covalently coupled to the surface of magnetic beads, and they are used to detect and isolate DogTag-fusion proteins efficiently.
Ligand: DogCatcher
Bead size: ~ 40 µm
Reactivity: Highly selective for DogTag-fusion proteins.
Binding Capacity: High binding capacity, 10 µL slurry bind about 10-20µg of DogTag-fusion protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300.
Background:
The DogCatcher-DogTag system was developed seven years ago as a method for protein ligation.
It is based on a modified domain from a S. pneumoniae surface protein (RrgA). Upon recognition, the two form a covalent isopeptide bond between the side chains of a lysine in DogCatcher and an Asparagine in DogTag. This technology has been used, among other applications, to create covalently stabilized multi-protein complexes, for modular vaccine production, and to label proteins. The DogTag system is versatile as the tag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins; similarly, DogCatcher can be fused to reporter proteins such as GFP, and to epitope or purification tags. DogTag/DogCatcher can be used for rapid loop-mediated labeling of ion channels in live mammalian cells.
Applications
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
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Telephone:
+86 138 8067 6215
Address:
Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130
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