mScarlet3-H/mYongHong Nanoselector Magnetic beads

mScarlet3-H/mYongHong Nanoselector Magnetic beads
mScarlet3-H/mYongHong Nanoselector Magnetic beads

Details and Advantages

Code: 028-101-003

Specification: 0.5 mL(20 rxns) / 1 mL(40 rxns)

Applications: IP,CHIP,MS

Reactivity: mScarlet

Conjugate: Magnetic beads

Advantages:

  • Consistent and reproducible results

  • No heavy & light antibody chains

  • Extraordinary binding, even under harsh conditions

  • High affinity

  • Short Incubation (5-30 min)

Product Detail

Product Detail

Description:
mScarlet3-H/mYongHong Nanoselector Magnetic beads have been specifically designed to bind mScarlet-fusion proteins. mScarlet3-H/mYongHong Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. mScarlet3-H/mYongHong Nanoselector Magnetic beads are ideal tools to isolate or purify mScarlet-fusion proteins fast and efficiently.

Ligand: Anti-mScarlet single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: mScarlet,mScarlet3,mScarlet3-H/mYongHong
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mScarlet.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300


Background:
The fluorescent protein mScarlet is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.
For biochemical analysis including mass spectrometry and enzyme activity measurements these mScarlet-fusion proteins and their interacting factors need to be isolated fast and efficiently by immunoprecipitation using the mScarlet Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.


Applications:

Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis

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