Anti-M13 VHH-HRP | Phage Display Detection Nanobody

Product Detail

Anti-M13 Bacteriophage, AlpSdAbs® VHH(HRP) – High-Affinity Recombinant Nanobody for Phage Detection

Phage display system is a powerful platform for selecting and screening antibody fragments, peptides, and proteins. Our Anti-M13 Bacteriophage AlpSdAbs® VHH(HRP) is a recombinant single-domain antibody (VHH) specifically designed for sensitive and high-specificity detection of M13 bacteriophage in phage display and related immunoassays. As an HRP-conjugated nanobody, it combines the superior selectivity of single-domain antibodies with the convenience of enzymatic detection, making it an ideal tool for ELISA, phage immunodetection, and screening applications.

Description:

Anti-M13 Bacteriophage, AlpSdAbs® VHH(HRP) is designed for detecting M13 bacteriophage specifically. Anti-M13 Bacteriophage, AlpSdAbs® VHH(HRP) is based on monoclonal, recombinant, single domain antibody to M13 bacteriophage coupled to HRP. Based on ELISA, Anti-M13 Bacteriophage, AlpSdAbs® VHH(HRP) reacts with the M13 bacteriophage selectively, no reactivity with other proteins.

Technical Specifications

Immunogen: Full length M13 phage coat protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: M13 phage coat protein, exact epitope not determined
Cross-Reactivity: Highly selective for M13 phage
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), protect from light

Key Features

High specificity: Targets M13 bacteriophage coat protein with no cross-reactivity to other proteins.
HRP-conjugated: Provides high signal sensitivity and direct detection in ELISA assays.
Recombinant VHH: Animal-free, batch-to-batch consistent, stable supply.
Compact single-domain antibody: <15 kDa, high permeability and rapid binding.
Versatile applications: Suitable for phage display screening, immunodetection of M13, and protein research.
Fast and reliable: Short incubation (5–30 min), consistent and reproducible results.

Applications

ELISA detection of M13 bacteriophage
Phage display library screening and binder selection
Phage immunoprecipitation (IP) and Co-IP
Recombinant protein purification
Phage-based diagnostics and research assays

Background

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6470 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with fi¬lamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance

ELISA: 1:10,000-1:50,000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Call to Action

Enhance your phage display assays and ELISA workflows with Anti-M13 Bacteriophage AlpSdAbs® VHH(HRP). Request a Quote or contact our technical support for experimental guidance and bulk orders.

FAQ

Citations