Description:
Anti-TIGIT, AlpHcAbs® Human antibody is designed for detecting human TIGIT specifically. Based on ELISA and/or FCM, Anti-TIGIT, AlpHcAbs® Human antibody reacts with human TIGIT specifically.
Immunogen: Recombinant human TIGIT
Host: Alpaca pacous
Isotype: Human IgG1
Conjugate: Unconjugated
Specificity: Human TIGIT
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)
Background:
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Application:
ELISA: 1:4,000-1:10000
Flow Cytometry: 1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.