Description:
Rat IgG2c Isotype Control is designed as a negative control in Rat IgG2c antibody staining applications. Rat IgG2c Isotype Control belongs to the same immunoglobulin class and subclass as the Rat IgG2c antibody which is used in the experiment but lack affinity to the protein of interest. Rat IgG2c Isotype Control has no antigen-binding capacity, and it makes experiments credible,
Isotype: Rat IgG2c(kappa)
Conjugate: Unconjugated
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5)
Storage: Store at -20 °C, (Avoid freeze / thaw cycles)
Background:
Antibodies bind to a specific protein or antigen, but sometimes an antibody can bind to other proteins or cellular components in a non-specific manner. Experimental controls are critical in determining if the antibodies used in your experiment have any off-target binding capabilities. Off-target or non-specific binding by antibodies lead to false positive results, making it difficult to determine background staining from antigen-specific staining. Therefore, it is important to always include experimental controls to ensure reliable results.
Application Notes:
Using at a dilution as the test antibody which is used in the experiment.