VHH Isotype Control, anti-KLH

Details and Advantages

Code: 400-101-016

Size: 1mg / 20mg

Applications: Isotype Control

Specificity:

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

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Product Description Manual MSDS FAQ Citations

Description:
VHH Isotype Control, anti-KLH is designed as a negative control in VHH antibody staining applications. VHH Isotype Control, anti-KLH has the same immunoglobulin class and subclass as the VHH antibody which is used in the experiment but lack affinity to the protein of interest. VHH Isotype Control, anti-KLH makes experiments credible.

Isotype: VHH domain of Alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag was added at the C terminal of the VHH)
Specificity: Keyhole Limpet Hemocyanin(KLH)
Purity: Human cell production, Recombinant Expression and Affinity purified, > 95%(HPLC)
Concentration: 1mg/ml
Endotoxin: < 1 EU/mg(LAL gel clotting)
Formation: Liquid, 10mM PBS (pH 7.5)
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)

Background:
Antibodies bind to a specific protein or antigen, but sometimes an antibody can bind to other proteins or cellular components in a non-specific manner. Experimental controls are critical in determining if the antibodies used in your experiment have any off-target binding capabilities. Off-target or non-specific binding by antibodies lead to false positive results, making it difficult to determine background staining from antigen-specific staining. Therefore, it is important to always include experimental controls to ensure reliable results.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Application Notes:

Negative control for VHH antibody
Using at a dilution as the antibody which is used in the experiment.

FAQ

Citations