Anti-GFP, AlpHcAbs® Rabbit antibody

Details and Advantages

Code: 019-201-001

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,Elisa,IP

Reactivity: GFP

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-GFP, AlpHcAbs® Rabbit antibody is designed for detecting GFP fusion protein specifically. Anti-GFP, AlpHcAbs® Rabbit antibody is monovalent, recombinant fab of alpaca IgG1 antibody fused to rabbit IgG Fc. Based on immunoelectrophoresis and/or ELISA, Anti-GFP, AlpHcAbs® Rabbit antibody is useful for detecting GFP fusion proteins with high sensitivity.

Immunogen: GFP
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to rabbit IgG Fc(mutation)
Conjugate: Unconjugated
Specificity: GFP
Cross-Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants. Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C(Avoid freeze / thaw cycles)

Background:
Green fluorescent proteins (GFPs) and the variants thereof are widely used to study protein function and location. GFP was originally derived from the jellyfish Aequorea victoria. It has 238 amino acid residues and a green fluorophore, which is comprised of only three amino acids: Ser65-Tyr66-Gly67. The stable protein structure is formed by beta sheets, which have a conformation that makes up an 11-stranded drum-like structure. The stability of GFP allows it to withstand pH levels ranging from mildly acidic (pH=5.5) to extremely basic (pH=12), and can also resist temperatures of up to 65°C. GFP has major and minor excitation peaks at wavelengths of 395 nm and 475 nm, respectively.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:10000-1:20000
ELISA: 1:10000-1:20000

ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 106 cells

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations