Anti-GST tag, AlpHcAbs® Mouse IgG1 antibody

Details and Advantages

Code: 010-301-001

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,Elisa,IP

Reactivity: GST

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-GST Tag, AlpHcAbs® Mouse IgG1 antibody is designed for detecting GST tag fusion proteins. Anti-GST Tag, AlpHcAbs® Mouse IgG1 antibody is monovalent, recombinant single domain antibody fused to mouse IgG1 Fc. Based on immunoelectrophoresis and/or ELISA, Anti-GST Tag, AlpHcAbs® Mouse IgG1 antibody detects the GST tag selectively, no reactivity with other proteins.

Immunogen: GST tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: GST tag
Cross-Reactivity: No cross-reactivity with other proteins
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: store at -20 °C. Avoid freeze / thaw cycle

Background:
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade. GST is one useful epitope ta for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:5000-1:20,000
ELISA: 1:5,000-20,000
IP: 1-2ug/sample

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations