Anti-Halo tag, AlpHcAbs® Mouse IgG1 antibody

Details and Advantages

Code: 012-301-001

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,Elisa,IP

Reactivity: Halo tag

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Halo Tag, AlpHcAbs® Mouse IgG1 antibody is designed for detecting Halo tag fusion proteins. Anti-Halo Tag, AlpHcAbs® Mouse IgG1 antibody is monovalent, recombinant single domain antibody fused to mouse IgG1 Fc. Based on immunoelectrophoresis and/or ELISA, Anti-Halo Tag, AlpHcAbs® Mouse IgG1 antibody detects the Halo tag selectively, no reactivity with other proteins.

Immunogen: Halo-KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: Halo tag
Cross-Reactivity: No cross-reactivity with other proteins
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: store at –20 °C. Avoid freeze / thaw cycle

Background:
The protein tag(Halo tag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (Halo tag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Due to the single-chain nature of sdAbs and their increased sensitivity and higher affinity to Halo tag fusion proteins, Anti-Halo Tag, AlpHcAbs® Mouse IgG1 antibody can be used to detect the Halo tag selectively, with high sensitivity and negligible non-specific background.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:5000-1:20,000
ELISA: 1:5,000-20,000

ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt: 1:200-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations