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Anti-mGold, AlpHcAbs® Rabbit IgG antibody

Anti-mGold, AlpHcAbs® Rabbit IgG antibody
Anti-mGold, AlpHcAbs® Rabbit IgG antibody
Details and Advantages

Code: 061-201-001

Size: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,ELISA,IP

Specificity: mGold

Conjugate: Unconjugated

Advantages:

  • High lot-to-lot consistency

  • Increased sensitivity and higher affinity

  • Animal-free production

Product Detail

Description:

Anti-mGold, AlpHcAbs® Rabbit IgG antibody is designed for detecting mGold fusion proteins specifically. Anti-mGold, AlpHcAbs® Rabbit IgG antibody is a monoclonal, recombinant, rabbit IgG Fc fused single domain antibody. Based on western blot and ELISA, Anti-mGold, AlpHcAbs® Rabbit IgG antibody detects the mGold selectively, no reactivity with other proteins.

Immunogen: mGold
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc
Conjugate: Unconjugated
Specificity: mGold, mGold2s, mGold2t
Cross-Reactivity: Recognizes GFP and YFP variants
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at -20 °C, (Avoid freeze / thaw cycles)


Background:

mGold is a yellow fluorescent protein (YFPs), and its variants mGold2s and mGold2t are the brightest and most photostable monomeric yellow fluorescent proteins, breaking the traditional trade-off between photostability and brightness and achieving simultaneous enhancement of both properties. Compared to widely used YFPs such as mVenus and mCitrine, their photostability has increased by 25 times, with minimal changes to other biophysical properties.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.


Applications

ELISA: 1:5000 -1:20000
WB: 1:5000 -1:20000
ICC/IF: 1:200-1:1000
IP: 2ug/sample
Flow Cyt: 1:200-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Telephone:

+86 138 8067 6215

Address:

Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130