Anti-mNeongreen, AlpHcAbs® Mouse IgG1 antibody

Details and Advantages

Code: 013-301-001

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,Elisa,IP

Reactivity: mNeongreen

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-mNeongreen, AlpHcAbs® Mouse IgG1 antibody is designed for detecting mNeongreen fusion protein specifically. Anti-mNeongreen, AlpHcAbs® Mouse IgG1 antibody is monovalent, recombinant single domain antibody fused to mouse IgG1 Fc. Based on immunoelectrophoresis and/or ELISA, Anti-mNeongreen, AlpHcAbs® Mouse IgG1 antibody is useful for detecting mNeongreen fusion proteins with high sensitivity.

Immunogen: mNeongreen
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: mNeongreen
Cross-Reactivity: Highly selective for mNeongreen
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
mNeongreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single molecule super-resolution imaging and is an excellent fluorescence resonance energy transfer(FRET) acceptor for the newest cyan fluorescent proteins.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:1,000-1:5000

ELISA: 1:5,000-1:20,000

ICC/IF: 1:200-1:1000

IP: 1-2ug/sample
Flow Cyt: 1:100-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations