Description:
Anti-mScarlet3-H, AlpHcAbs® Rabbit antibody(Biotin) is designed for detecting mScarlet fusion proteins specifically. Anti-mScarlet3-H, AlpHcAbs® Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to mScarlet coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-mScarlet3-H, AlpHcAbs® Rabbit antibody(Biotin) detects the mScarlet, no reactivity with other proteins.
Immunogen: mScarlet
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc(mutation)
Conjugate: Biotin
Specificity: mScarlet,mScarlet3,mScarlet3-H
Cross-Reactivity: Highly selective for mScarlet
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C(Avoid freeze / thaw cycles), protect from light
Background:
The fluorescent protein mScarlet is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Performance
WB: 1:10,000-1:50,000
ELISA: 1:10,000-1:50,000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.