Description:
Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody(PE) is designed for detecting Myc tag fusion proteins specifically. Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody(PE) is based on monoclonal, recombinant, mouse IgG2a Fc fused fab of alpaca IgG1 antibody to Myc tag fused to PE, and Anti-Myc tag, AlpHcAbs® Mouse IgG2a antibody(PE) detects the Myc tag selectively, no reactivity with other proteins.
Immunogen: Myc tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to mouse IgG2a Fc(mutation)
Conjugate: PE(Ex: 488nm, Em: 575nm)
Specificity: Myc tag sequence(EQKLISEEDL)
Cross-Reactivity: Highly selective for Myc tag sequence
Purity: Recombinant expression and Affinity purified
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at 2-8 °C, Protect from light.
Background:
The Myc peptide are widely used for detecting or manipulating proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Performance
Flow Cyt: 1:200-1:1000
ICC/IF: 1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.