Anti-RFP, AlpHcAbs® Rabbit antibody
Code: 020-201-001
Specification: 100ug / 1mg
Applications: WB,ELISA,IP
Reactivity: RFP
Conjugate: Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Product Detail
Description:
Anti-RFP, AlpHcAbs® Rabbit antibody is designed for detecting RFP fusion protein specifically. Anti-RFP, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-RFP, AlpHcAbs® Rabbit antibody is useful for detecting RFP fusion proteins with high sensitivity.
Immunogen: RFP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Unconjugated
Specificity: RFP
Cross-Reactivity: Recognizes mCherry, mRFP, mScarlet, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at -20 °C(Avoid freeze / thaw cycles)
Performance
ELISA: 1:5,000-1:20,000
WB: 1:5000 -1:20000
IP: 1:100-1:500
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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Telephone:
+86 138 8067 6215
Address:
Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130
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Western blot-Anti-RFP, AlpHcAbs® Rabbit antibody(020-201-001)
Lane1-6: different amount(200ng-100ng-50ng-25ng-12.5-6.25ng) of mCherry was loaded. First antibody:Anti-RFP, AlpHcAbs® Rabbit antibody(020-201-001) at 0.1 µg/ml(1:10000) Secondary antibody： Anti-Rabbit IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP)(025-401-005)(1:10000)
