Anti-StayGold/mBaojin, AlpHcAbs® Mouse IgG1 antibody

Details and Advantages

Code: 082-301-001

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,Elisa,IP

Reactivity: StayGold/mBaojin

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-StayGold/mBaojin, AlpHcAbs® Mouse IgG1 antibody is designed for detecting StayGold fusion protein specifically. Anti-StayGold/mBaojin, AlpHcAbs® Mouse IgG1 antibody is monovalent, recombinant single domain antibody fused to mouse IgG1 Fc. Based on western blot and ELISA, Anti-StayGold/mBaojin, AlpHcAbs® Mouse IgG1 antibody reacts with the StayGold selectively, no reactivity with other proteins.

Immunogen: StayGold fusion protein
Clone No: A2F7
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: StayGold
Cross-Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C, (Avoid freeze / thaw cycles)

Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imagingUsing antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:5,000-1:20000

ELISA: 1:5,000-1:20000

ICC/IF: 1:100-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 10⁶ cells

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations